Influence of Aging on Biological Properties of Periodontal Ligament Cells

dc.creatorBenatti, BB
dc.creatorSilverio, KG
dc.creatorCasati, MZ
dc.creatorSallum, EA
dc.creatorNociti, FH
dc.date2008
dc.date2014-11-19T13:40:38Z
dc.date2015-11-26T17:06:27Z
dc.date2014-11-19T13:40:38Z
dc.date2015-11-26T17:06:27Z
dc.date.accessioned2018-03-28T23:54:54Z
dc.date.available2018-03-28T23:54:54Z
dc.identifierConnective Tissue Research. Taylor & Francis Ltd, v. 49, n. 6, n. 401, n. 408, 2008.
dc.identifier0300-8207
dc.identifierWOS:000261653800003
dc.identifier10.1080/03008200802171159
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60200
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60200
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60200
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279857
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabolism and growth-associated factors. PDLC cultures were obtained from subjects aged. 15 to 20 years and subjects aged more than 60 years. Proliferation; cell viability, mineralization assays, and mRNA levels were assessed for type I and III collagen, platelet-derived growth factor (PDGF)-1, basic fibroblast growth factor (bFGF), metalloproteinase (MMP)-2 and -8, and tissue inhibitor of metalloproteinases (TIMP)-1 and -2. Data analysis demonstrated that aging negatively influenced cell proliferation and mineral nodule formation (p < 0.05). Gene expression analysis further showed that mRNA levels for bFGF, PDGF-1, and TIMP-2 were not affected by aging (p > 0.05). In addition, mRNA levels for type I and fit collagen were significantly lower in aged cells (p < 0.05), whereas MMP-2 and -8 and TIMP-I mRNA levels were higher (p < 0.05). Within the limits of the present study, data analysis suggests that aging-modulates important biological properties of periodontal ligament cells; diminishes the potential for mineral nodule formation, and favors extracellular matrix degradation.
dc.description49
dc.description6
dc.description401
dc.description408
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionNational Research Council [305471/2006-6]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [04/14362-2, 04/14361-6]
dc.descriptionNational Research Council [305471/2006-6]
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationConnective Tissue Research
dc.relationConnect. Tissue Res.
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectAging
dc.subjectPeriodontal Ligament
dc.subjectCells
dc.subjectGene Expression
dc.subjectPeriodontal Heaing
dc.subjectFibroblast-growth-factor
dc.subjectIn-vitro
dc.subjectMatrix Metalloproteinases
dc.subjectReplicative Senescence
dc.subjectAlkaline-phosphatase
dc.subjectGingival Fibroblasts
dc.subjectDown-regulation
dc.subjectExpression
dc.subjectTissue
dc.subjectAge
dc.titleInfluence of Aging on Biological Properties of Periodontal Ligament Cells
dc.typeArtículos de revistas


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