Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family

dc.creatorMotta, MCM
dc.creatorMartins, ACD
dc.creatorde Souza, SS
dc.creatorCatta-Preta, CMC
dc.creatorSilva, R
dc.creatorKlein, CC
dc.creatorde Almeida, LGP
dc.creatorCunha, OD
dc.creatorCiapina, LP
dc.creatorBrocchi, M
dc.creatorColabardini, AC
dc.creatorLima, BD
dc.creatorMachado, CR
dc.creatorSoares, CMD
dc.creatorProbst, CM
dc.creatorde Menezes, CBA
dc.creatorThompson, CE
dc.creatorBartholomeu, DC
dc.creatorGradia, DF
dc.creatorPavoni, DP
dc.creatorGrisard, EC
dc.creatorFantinatti-Garboggini, F
dc.creatorMarchini, FK
dc.creatorRodrigues-Luiz, GF
dc.creatorWagner, G
dc.creatorGoldman, GH
dc.creatorFietto, JLR
dc.creatorElias, MC
dc.creatorGoldman, MHS
dc.creatorSagot, MF
dc.creatorPereira, M
dc.creatorStoco, PH
dc.creatorde Mendonca-Neto, RP
dc.creatorTeixeira, SMR
dc.creatorMaciel, TEF
dc.creatorMendes, TAD
dc.creatorUrmenyi, TP
dc.creatorde Souza, W
dc.creatorSchenkman, S
dc.creatorde Vasconcelos, ATR
dc.date2013
dc.dateAPR 3
dc.date2014-08-01T18:34:22Z
dc.date2015-11-26T17:06:14Z
dc.date2014-08-01T18:34:22Z
dc.date2015-11-26T17:06:14Z
dc.date.accessioned2018-03-28T23:54:41Z
dc.date.available2018-03-28T23:54:41Z
dc.identifierPlos One. Public Library Science, v. 8, n. 4, 2013.
dc.identifier1932-6203
dc.identifierWOS:000318840100054
dc.identifier10.1371/journal.pone.0060209
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80894
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80894
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279805
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionEndosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. In an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. The monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. The monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. The assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.
dc.description8
dc.description4
dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionERC AdG SISYPHE
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectMannose-type Oligosaccharides
dc.subjectBacterial-cell Division
dc.subjectAedes-aegypti Midgut
dc.subjectCrithidia-deanei
dc.subjectEscherichia-coli
dc.subjectKinetoplastid Parasites
dc.subjectBlastocrithidia-culicis
dc.subjectProteobacterial Endosymbionts
dc.subjectBearing Trypanosomatids
dc.subjectInsect Trypanosomatids
dc.titlePredicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family
dc.typeArtículos de revistas


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