Measuring the antioxidant capacity of blood plasma using potentiometry

dc.creatorTessutti, LS
dc.creatorMacedo, DV
dc.creatorKubota, LT
dc.creatorAlves, AA
dc.date2013
dc.dateOCT 15
dc.date2014-07-30T13:40:48Z
dc.date2015-11-26T17:06:10Z
dc.date2014-07-30T13:40:48Z
dc.date2015-11-26T17:06:10Z
dc.date.accessioned2018-03-28T23:54:36Z
dc.date.available2018-03-28T23:54:36Z
dc.identifierAnalytical Biochemistry. Academic Press Inc Elsevier Science, v. 441, n. 2, n. 109, n. 114, 2013.
dc.identifier0003-2697
dc.identifierWOS:000324605400004
dc.identifier10.1016/j.ab.2013.07.012
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53463
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53463
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279784
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n = 60) diluted (0.3 to 1 ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (Delta E). Delta E presented a good inverse correlation with added increasing concentrations of ascorbate (2.5-75 mu mol/L; R = -0.99), urate (9.0-150 mu mol/L; R = -0.99), and bilirubin (0.78-13 mu mol/L; R = -0.99). Increase in the antioxidant capacity decreased Delta E. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5-50 mu mol/L) presented a direct correlation (0.97) with Delta E. Furthermore, Delta E presented an inverse correlation (R = -0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5-75 mu mol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n = 15; exercised, n = 15). This exercise decreased the concentration of urate (p < 0.05), decreased FRAP (p < 0.5), increased TBARS (p < 0.5), and decreased the potentiometer sensor response (p = 6.5 x 10(-3)). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples. (c) 2013 Elsevier Inc. All rights reserved.
dc.description441
dc.description2
dc.description109
dc.description114
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [10/02791-7]
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationAnalytical Biochemistry
dc.relationAnal. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectPotentiometry
dc.subjectTotal antioxidant capacity
dc.subjectPlasma
dc.subjectExhaustive exercise
dc.subjectOxidative-stress
dc.subjectCyclic Voltammetry
dc.subjectSkeletal-muscle
dc.subjectRat-liver
dc.subjectAmperometric Sensor
dc.subjectLipid-peroxidation
dc.subjectBiological-fluids
dc.subjectAscorbic-acid
dc.subjectExercise
dc.subjectElectrode
dc.titleMeasuring the antioxidant capacity of blood plasma using potentiometry
dc.typeArtículos de revistas


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