Transcriptional Analysis of Drought-Induced Genes in the Roots of a Tolerant Genotype of the Common Bean (Phaseolus vulgaris L.)

dc.creatorRecchia, GH
dc.creatorCaldas, DGG
dc.creatorBeraldo, ALA
dc.creatorda Silva, MJ
dc.creatorTsai, SM
dc.date2013
dc.dateAPR
dc.date2014-08-01T18:20:47Z
dc.date2015-11-26T17:06:10Z
dc.date2014-08-01T18:20:47Z
dc.date2015-11-26T17:06:10Z
dc.date.accessioned2018-03-28T23:54:35Z
dc.date.available2018-03-28T23:54:35Z
dc.identifierInternational Journal Of Molecular Sciences. Mdpi Ag, v. 14, n. 4, n. 7155, n. 7179, 2013.
dc.identifier1422-0067
dc.identifierWOS:000318017100030
dc.identifier10.3390/ijms14047155
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77516
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77516
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279780
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionIn Brazil, common bean (Phaseolus vulgaris L.) productivity is severely affected by drought stress due to low technology cultivation systems. Our purpose was to identify differentially expressed genes in roots of a genotype tolerant to water deficit (BAT 477) when submitted to an interruption of irrigation during its development. A SSH library was constructed taking as 'driver' the genotype Carioca 80SH (susceptible to drought). After clustering and data mining, 1572 valid reads were obtained, resulting in 1120 ESTs (expressed sequence tags). We found sequences for transcription factors, carbohydrates metabolism, proline-rich proteins, aquaporins, chaperones and ubiquitins, all of them organized according to their biological processes. Our suppressive subtractive hybridization (SSH) library was validated through RT-qPCR experiment by assessing the expression patterns of 10 selected genes in both genotypes under stressed and control conditions. Finally, the expression patterns of 31 ESTs, putatively related to drought responses, were analyzed in a time-course experiment. Our results confirmed that such genes are more expressed in the tolerant genotype during stress; however, they are not exclusive, since different levels of these transcripts were also detected in the susceptible genotype. In addition, we observed a fluctuation in gene regulation over time for both the genotypes, which seem to adopt and adapt different strategies in order to develop tolerance against this stress.
dc.description14
dc.description4
dc.description7155
dc.description7179
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCNPq [Universal-474337/2008-1]
dc.languageen
dc.publisherMdpi Ag
dc.publisherBasel
dc.publisherSuíça
dc.relationInternational Journal Of Molecular Sciences
dc.relationInt. J. Mol. Sci.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectPhaseolus vulgaris L.
dc.subjectdrought stress
dc.subjectroot
dc.subjectsuppressive subtractive hybridization library
dc.subjectgene expression
dc.subjectStress Tolerance
dc.subjectWater-deficit
dc.subjectPlant-responses
dc.subjectMessenger-rnas
dc.subjectArabidopsis
dc.subjectIdentification
dc.subjectAccumulation
dc.subjectResistance
dc.subjectNutrition
dc.subjectProteins
dc.titleTranscriptional Analysis of Drought-Induced Genes in the Roots of a Tolerant Genotype of the Common Bean (Phaseolus vulgaris L.)
dc.typeArtículos de revistas


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