A new amperometric biosensor for fructose using a carbon paste electrode modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase

dc.creatorGarcia, CAB
dc.creatorNeto, GD
dc.creatorKubota, LT
dc.creatorGrandin, LA
dc.date1996
dc.date44501
dc.date2014-12-16T11:34:40Z
dc.date2015-11-26T17:05:10Z
dc.date2014-12-16T11:34:40Z
dc.date2015-11-26T17:05:10Z
dc.date.accessioned2018-03-28T23:53:30Z
dc.date.available2018-03-28T23:53:30Z
dc.identifierJournal Of Electroanalytical Chemistry. Elsevier Science Sa Lausanne, v. 418, n. 41671, n. 147, n. 151, 1996.
dc.identifier0022-0728
dc.identifierWOS:A1996WA97600019
dc.identifier10.1016/S0022-0728(96)04775-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76137
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/76137
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76137
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279514
dc.descriptionA carbon paste modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase was used to construct a new biosensor for fructose. An efficient electron transfer mediation from the reduced enzyme to the carbon paste electrode was verified, at a low applied potential of 20mV vs. SCE, where interfering reactions are minimized. A linear response range for 0.1 to 0.8 mmol(-1) fructose was adjusted by the equation I=0.0150(+/-0.006)+0.618(+/-0.011) [fructose], with a correlation coefficient of 0.999 and an rsd of 0.68% for n=7. The biosensor was used to determine fructose in sweets and fruit jellies and the results present good recovery, higher than 96% in both cases. A stable response is observed over two months, when stored in a refrigerator, or for 300 measurements in continuous use.
dc.description418
dc.description41671
dc.description147
dc.description151
dc.languageen
dc.publisherElsevier Science Sa Lausanne
dc.publisherLausanne
dc.publisherSuíça
dc.relationJournal Of Electroanalytical Chemistry
dc.relationJ. Electroanal. Chem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectbiosensors
dc.subjectfructose
dc.subjectMeldola's Blue mediator
dc.subjectmodified silica gel
dc.subjectfructose 5-dehydrogenase
dc.subjectFlow-injection Analysis
dc.subjectGlucose
dc.subjectEnzyme
dc.subjectDehydrogenase
dc.subjectChromatography
dc.subjectMixtures
dc.subjectPlatinum
dc.subjectMediator
dc.subjectSucrose
dc.subjectSurface
dc.titleA new amperometric biosensor for fructose using a carbon paste electrode modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase
dc.typeArtículos de revistas


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