Determination of levocetirizine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: Application to a bioequivalence study

dc.creatorMorita, MR
dc.creatorBerton, D
dc.creatorBoldin, R
dc.creatorBarros, FAP
dc.creatorMeurer, EC
dc.creatorAmarante, AR
dc.creatorCampos, DR
dc.creatorCalafatti, SA
dc.creatorPereira, R
dc.creatorAbib, E
dc.creatorPedrazolli, J
dc.date2008
dc.dateFEB 1
dc.date2014-11-19T08:58:53Z
dc.date2015-11-26T17:04:31Z
dc.date2014-11-19T08:58:53Z
dc.date2015-11-26T17:04:31Z
dc.date.accessioned2018-03-28T23:52:48Z
dc.date.available2018-03-28T23:52:48Z
dc.identifierJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 862, n. 41671, n. 132, n. 139, 2008.
dc.identifier1570-0232
dc.identifierWOS:000253354200015
dc.identifier10.1016/j.chromb.2007.11.028
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61398
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61398
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61398
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279369
dc.descriptionWe describe a liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for levocetirizine quantification (I) in human plasma. Sample preparation was made using a fexofenadine (H) addition as internal standard (IS), liquid-liquid extraction using cold dichloromethane, and dissolving the final extract in acetonitrile. I and II (IS) were injected in a C18 column and the mobile phase composed of acetonitrile:water:formic acid (80.00:19.90:0.10, v/v/v) and monitored using positive electrospray source with tandem mass spectrometry analyses. The selected reaction monitoring (SRM) was set using precursor ion and product ion combinations of m/z 389 > 201 forI and m/z 502 > 467 for II. The limit of quantification and the dynamic range achieved were 0.5 ng/mL and 0.5-500.0 ng/mL. Validation results on linearity, specificity, accuracy, precision and stability, as well as its application to the analysis of plasma samples taken up to 48 h after oral administration of 5 mg of levocetirizine dichloridrate in healthy volunteers demonstrate its applicability to bioavailability studies. (c) 2007 Elsevier B.V. All rights reserved.
dc.description862
dc.description41671
dc.description132
dc.description139
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences
dc.relationJ. Chromatogr. B
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectmass spectrometry
dc.subjectbioavailability
dc.subjectlevocetirizine
dc.subjectAllergic Rhinitis
dc.subjectCetirizine
dc.titleDetermination of levocetirizine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: Application to a bioequivalence study
dc.typeArtículos de revistas


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