Neutrophil migration in mice induced by a mannose-binding lectin isolated from Annona coriacea seeds

dc.creatorCoelho, MB
dc.creatorDeSouza, IA
dc.creatorFreire, MGM
dc.creatorMarangoni, S
dc.creatorAntunes, E
dc.creatorMacedod, MLR
dc.date2006
dc.dateOCT
dc.date2014-11-19T08:59:41Z
dc.date2015-11-26T17:04:31Z
dc.date2014-11-19T08:59:41Z
dc.date2015-11-26T17:04:31Z
dc.date.accessioned2018-03-28T23:52:48Z
dc.date.available2018-03-28T23:52:48Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 48, n. 5, n. 529, n. 535, 2006.
dc.identifier0041-0101
dc.identifierWOS:000241328700006
dc.identifier10.1016/j.toxicon.2006.07.001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65334
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65334
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65334
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279368
dc.descriptionA novel 14-kDa lectin from Annona coriacea seeds (ACLEC) with hemagglutinating activity on erythrocytes has been recently described. Since plant lectins are known to present inflammatory activity, this study aimed to investigate the leukocyte migration induced by ACLEC, and inflammatory mediators involved in this phenomenon. Mate Swiss mice were intraperitoneally injected with ACLEC (3-100 mu g/cavity), and at 4-96 h thereafter the leukocyte counts in peritoneal lavage fluid were evaluated. ACLEC induced a dose-dependent neutrophil accumulation, reaching maximal responses at 16 h after injection (approximately 40-fold increase for 30 mu g/cavity). Significant accumulation of mononuclear cells was observed at 72 h (2.7-fold increase). The carbohydrate mannose nearly abolished the neutrophil influx, whereas sucrose, glucose and galactose had no effect. Dexamethasone, the cyclooxygenase-2 (COX-2) inhibitor celecoxib and the Platelet activating factor (PAF) receptor antagonist PCA4248 significantly reduced ACLEC-induced neutrophil influx. The tachykinin NK1 antagonist SR140333, the tachykinin NK2 antagonist SR48968, the non-selective NO inhibitor L-NAME, the selective inducible NOS inhibitor aminoguanidine and the lypoxygenase inhibitor AA861 all failed to modify the ACLEC-induced responses. In conclusion, ACLEC is able to attract neutrophils into the mice peritoneal cavity by mechanisms involving interactions of the lectin with cell-specific mannose recognition, leading to the release of COX-2-derived mediators and PAF. (c) 2006 Elsevier Ltd. All rights reserved.
dc.description48
dc.description5
dc.description529
dc.description535
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAnnona coriacea lectin
dc.subjectneutrophil migration
dc.subjectinflammation
dc.subjectIn-vivo
dc.subjectNitric-oxide
dc.subjectLymphocyte-activation
dc.subjectPlant-lectins
dc.subjectPaw
dc.subjectInflammation
dc.subjectAgglutinin
dc.subjectReceptors
dc.subjectMechanism
dc.subjectCells
dc.titleNeutrophil migration in mice induced by a mannose-binding lectin isolated from Annona coriacea seeds
dc.typeArtículos de revistas


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