Determination of propranolol enantiomers in plasma and urine by spectrofluorimetry and second-order standard addition method

dc.creatorValderrama, P
dc.creatorPoppi, RJ
dc.date2009
dc.dateSEP 28
dc.date2014-11-19T07:59:29Z
dc.date2015-11-26T17:04:01Z
dc.date2014-11-19T07:59:29Z
dc.date2015-11-26T17:04:01Z
dc.date.accessioned2018-03-28T23:52:14Z
dc.date.available2018-03-28T23:52:14Z
dc.identifierAnalytica Chimica Acta. Elsevier Science Bv, v. 651, n. 1, n. 31, n. 35, 2009.
dc.identifier0003-2670
dc.identifierWOS:000270104800004
dc.identifier10.1016/j.aca.2009.08.003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61413
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61413
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61413
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279244
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe determination of propranolol enantiomers in human plasma and urine by spectrofluorimetry and a second-order standard addition method is described. The methodology is based on chiral recognition of propranolol by formation of an inclusion complex with beta-cyclodextrin, a chiral auxiliary, in the presence of I-butanol. The adopted strategy combines the use of PARAFAC, for extraction of the pure analyte signal, with the standard addition method, for determinations in the presence of an individual matrix effect caused by the quenching action of the proteins present in the plasma and urine. A specific PARAFAC model was built for each sample, in triplicate, and the scores were related to (R)-propranolol mole fraction using a linear regression in the standard addition method. Using a propranolol with concentration of 260 ng mL(-1), good results were obtained for determinations in the mole fraction range from 50 to 80% of (R)-propranolol, providing absolute errors between 0.4 and 3.6% for plasma and between 0.9 and 6.0% for urine. (C) 2009 Elsevier B.V. All rights reserved.
dc.description651
dc.description1
dc.description31
dc.description35
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [05/56188-1, 06/07309-3]
dc.descriptionCNPq [309992/2006-0]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationAnalytica Chimica Acta
dc.relationAnal. Chim. Acta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectPropranolol
dc.subjectAnantiomers
dc.subjectHuman plasma
dc.subjectUrine
dc.subjectSecond-order standard addition method
dc.subjectPerformance Liquid-chromatography
dc.subjectFluorescence Detection
dc.subjectChiral Recognition
dc.subjectCyclodextrin
dc.subjectCalibration
dc.subjectSeparation
dc.subjectCyclosophoraoses
dc.subject(r)-propranolol
dc.subject(s)-propranolol
dc.subjectSpectrometry
dc.titleDetermination of propranolol enantiomers in plasma and urine by spectrofluorimetry and second-order standard addition method
dc.typeArtículos de revistas


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