NEUTROPHILS AND MONONUCLEAR-CELLS FROM PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE RELEASE NITRIC-OXIDE

dc.creatorCONDINONETO, A
dc.creatorMUSCARA, MN
dc.creatorGRUMACH, AS
dc.creatorCARNEIROSAMPAIO, MMS
dc.creatorDENUCCI, G
dc.date1993
dc.dateMAY
dc.date2014-12-16T11:34:25Z
dc.date2015-11-26T17:03:57Z
dc.date2014-12-16T11:34:25Z
dc.date2015-11-26T17:03:57Z
dc.date.accessioned2018-03-28T23:52:09Z
dc.date.available2018-03-28T23:52:09Z
dc.identifierBritish Journal Of Clinical Pharmacology. Blackwell Science Ltd, v. 35, n. 5, n. 485, n. 490, 1993.
dc.identifier0306-5251
dc.identifierWOS:A1993LB06200008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65336
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65336
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65336
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279223
dc.description1 Chronic granulomatous disease (CGD) is a group of genetic disorders characterised by recurrent severe suppurative infections due to impaired microbial killing. The principal biochemical defect is an impairment in the production of reactive oxygen intermediates by phagocytes. 2 Nitric oxide (NO) is synthesised from the guanidino nitrogen atom(S) Of L-arginine and has recently been proposed to be involved in defence mechanisms. The aim of this study was to investigate the involvement of the oxidative burst in the biosynthesis of NO by neutrophils and mononuclear cells from patients with CGD. 3 NO synthesis was assayed by the ability of neutrophils and mononuclear cells to inhibit thrombin-induced washed platelet aggregation while superoxide anion (O2-) production was measured spectrophotometrically by the superoxide dismutase inhibitable reduction of cytochrome c. 4 Neutrophils and mononuclear cells from patients with CGD released NO. This release was inhibited by nitro-L-arginine methyl ester but could be reversed by L-arginine. Zymosan- and PMA-induced O2- production was less than 10% as compared with healthy controls. 5 These results indicate that O2- production is not essential for NO synthesis in human leucocytes.
dc.description35
dc.description5
dc.description485
dc.description490
dc.languageen
dc.publisherBlackwell Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationBritish Journal Of Clinical Pharmacology
dc.relationBr. J. Clin. Pharmacol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectENDOTHELIAL-DERIVED RELAXING FACTOR
dc.subjectSUPEROXIDE ANION
dc.subjectPLATELET AGGREGATION
dc.subjectRESPIRATORY BURST
dc.subjectHUMAN
dc.subjectL-arginine
dc.subjectRespiratory Burst
dc.subjectInterferon-gamma
dc.subjectRelaxing Factor
dc.subjectSuperoxide Production
dc.subjectMurine Macrophages
dc.subjectNitrate
dc.subjectIdentification
dc.subjectGranulocytes
dc.subjectAggregation
dc.titleNEUTROPHILS AND MONONUCLEAR-CELLS FROM PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE RELEASE NITRIC-OXIDE
dc.typeArtículos de revistas


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