Determination of primary structure of two isoforms 6-1 and 6-2 PLA(2) D49 from Bothrops jararacussu snake venom and neurotoxic characterization using in vitro neuromuscular preparation

dc.creatorPonce-Soto, LA
dc.creatorBonfim, VL
dc.creatorRodrigues-Simioni, L
dc.creatorNovello, JC
dc.creatorMarangoni, S
dc.date2006
dc.dateFEB
dc.date2014-11-19T07:42:54Z
dc.date2015-11-26T17:03:43Z
dc.date2014-11-19T07:42:54Z
dc.date2015-11-26T17:03:43Z
dc.date.accessioned2018-03-28T23:51:53Z
dc.date.available2018-03-28T23:51:53Z
dc.identifierProtein Journal. Springer, v. 25, n. 2, n. 147, n. 155, 2006.
dc.identifier1572-3887
dc.identifierWOS:000239249100006
dc.identifier10.1007/s10930-006-0006-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61412
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61412
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61412
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279154
dc.descriptionIn this paper we reported the purification, the biological characterization and the amino acid sequence of two new isoforms basic 6-1 (Bj-IV) and 6-2 (Bj-V) PLA(2) D49 purified from the Bothrops jararacussu venom The isoforms 6-1 and 6-2 had a sequence of amino acids of 121 amino acid residues 6-1: DLFEWGQMIL KETGKNPFPY YGAYGCYCGW GGRGKPKDKD TDRCCYVHDC CYKKLTGCPK TDDRYSYSWL DLTIVCGEDD PCKELCECDK AIAVCFRENL GTYNKKYRYH LKPCKKADKP C and pI value 7.83 and 6-2: DLWQFGQMIL KETGKIPFPY YGAYGCYCGW GGRGGKPKDG TDRCCYVHDC CYKKLTGCPK TDDRYSYSWL DLTIVCGEDD PCKELCECDK AIAVCFRENL GTYNKKYRYH LKPCKKADKP C with a pI value of 7.99 Skeletal muscle preparations from the young chicken have been used previously in order to study the effects of toxins on neuromuscular transmission, providing an important opportunity to study the differentiated behavior of a toxin before more than one model, because it shows differences in its sensibilities Both isoforms have produced neuromuscular blockade in young chicken biventer cervicis nerve-muscle preparations in presence or absence of crotapotin crotalic (F3 and F4) indicating that catalytic activity was not essential for neuromuscular action in this preparation.
dc.description25
dc.description2
dc.description147
dc.description155
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationProtein Journal
dc.relationProtein J.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectphospholipase A(2)
dc.subjectsnake venom
dc.subjectBothrops jararacussu
dc.subjectneuromuscular preparations
dc.subjectneurotoxic characterization
dc.subjectPhospholipase A(2) Enzymes
dc.subjectAmino-acid-sequence
dc.subjectNerve-muscle Preparation
dc.subjectEnzymatic Characterization
dc.subjectPiratoxin-ii
dc.subjectRegion
dc.subjectMyotoxicity
dc.subjectEvolution
dc.subjectInsights
dc.subjectHomolog
dc.titleDetermination of primary structure of two isoforms 6-1 and 6-2 PLA(2) D49 from Bothrops jararacussu snake venom and neurotoxic characterization using in vitro neuromuscular preparation
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución