dc.creatorRocco, SA
dc.creatorVelho, JA
dc.creatorMarin, RM
dc.creatorRolim, LD
dc.creatorVercesi, AE
dc.creatorRittner, R
dc.creatorFranchini, KG
dc.date2005
dc.date45717
dc.date2014-11-19T06:34:09Z
dc.date2015-11-26T17:03:20Z
dc.date2014-11-19T06:34:09Z
dc.date2015-11-26T17:03:20Z
dc.date.accessioned2018-03-28T23:51:27Z
dc.date.available2018-03-28T23:51:27Z
dc.identifierJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 817, n. 2, n. 297, n. 302, 2005.
dc.identifier1570-0232
dc.identifierWOS:000226957700022
dc.identifier10.1016/j.jchromb.2004.12.021
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68350
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/68350
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68350
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279049
dc.descriptionThe quinazoline derivative, 4-N-(3'-bromo-phenyl)amino-6,7-dimethoxyquinazoline (PD153035), has recently been identified as a potential drug for the treatment of proliferative disease. Here, we report a sensitive high performance liquid chromatography (HPLC)-based quantitative detection method for measurement of PD153035 levels in rat plasma. Sample pretreatment involved a two-step extraction with chloroform. The analytes were separated on a column packed with OmniSpher C18 material and eluted with acetonitrile-0.1 M ammonium acetate, pH 7.2 (70:30, v/v). The column effluent was monitored by UV detection at 330 nm. A linear response was achieved over the concentration range 0.50-100.00 muM using multilevel calibration with an internal standard. The analytical method inter- and intra-run accuracy and precision were better than +/-15%. The lower limit of quantification was 0.50 muM. The method has been applied to study the preclinical pharmacokinetics of this compound in rats. (C) 2004 Elsevier B.V. All rights reserved.
dc.description817
dc.description2
dc.description297
dc.description302
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences
dc.relationJ. Chromatogr. B
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subject4-anilinoquinazoline derivatives
dc.subjectliquid-liquid extraction
dc.subjecthigh performance liquid chromatography
dc.subjectMast-cell Inhibitor
dc.subjectIrreversible Inhibitors
dc.subjectCancer-chemotherapy
dc.subjectFamily
dc.titleHigh performance liquid chromatography analysis of a 4-anilinoquinazoline derivative (PD153035), a specific inhibitor of the epidermal growth factor receptor tyrosine kinase, in rat plasma
dc.typeArtículos de revistas


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