dc.creator | Rocco, SA | |
dc.creator | Velho, JA | |
dc.creator | Marin, RM | |
dc.creator | Rolim, LD | |
dc.creator | Vercesi, AE | |
dc.creator | Rittner, R | |
dc.creator | Franchini, KG | |
dc.date | 2005 | |
dc.date | 45717 | |
dc.date | 2014-11-19T06:34:09Z | |
dc.date | 2015-11-26T17:03:20Z | |
dc.date | 2014-11-19T06:34:09Z | |
dc.date | 2015-11-26T17:03:20Z | |
dc.date.accessioned | 2018-03-28T23:51:27Z | |
dc.date.available | 2018-03-28T23:51:27Z | |
dc.identifier | Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 817, n. 2, n. 297, n. 302, 2005. | |
dc.identifier | 1570-0232 | |
dc.identifier | WOS:000226957700022 | |
dc.identifier | 10.1016/j.jchromb.2004.12.021 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68350 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/68350 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/68350 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1279049 | |
dc.description | The quinazoline derivative, 4-N-(3'-bromo-phenyl)amino-6,7-dimethoxyquinazoline (PD153035), has recently been identified as a potential drug for the treatment of proliferative disease. Here, we report a sensitive high performance liquid chromatography (HPLC)-based quantitative detection method for measurement of PD153035 levels in rat plasma. Sample pretreatment involved a two-step extraction with chloroform. The analytes were separated on a column packed with OmniSpher C18 material and eluted with acetonitrile-0.1 M ammonium acetate, pH 7.2 (70:30, v/v). The column effluent was monitored by UV detection at 330 nm. A linear response was achieved over the concentration range 0.50-100.00 muM using multilevel calibration with an internal standard. The analytical method inter- and intra-run accuracy and precision were better than +/-15%. The lower limit of quantification was 0.50 muM. The method has been applied to study the preclinical pharmacokinetics of this compound in rats. (C) 2004 Elsevier B.V. All rights reserved. | |
dc.description | 817 | |
dc.description | 2 | |
dc.description | 297 | |
dc.description | 302 | |
dc.language | en | |
dc.publisher | Elsevier Science Bv | |
dc.publisher | Amsterdam | |
dc.publisher | Holanda | |
dc.relation | Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences | |
dc.relation | J. Chromatogr. B | |
dc.rights | fechado | |
dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.source | Web of Science | |
dc.subject | 4-anilinoquinazoline derivatives | |
dc.subject | liquid-liquid extraction | |
dc.subject | high performance liquid chromatography | |
dc.subject | Mast-cell Inhibitor | |
dc.subject | Irreversible Inhibitors | |
dc.subject | Cancer-chemotherapy | |
dc.subject | Family | |
dc.title | High performance liquid chromatography analysis of a 4-anilinoquinazoline derivative (PD153035), a specific inhibitor of the epidermal growth factor receptor tyrosine kinase, in rat plasma | |
dc.type | Artículos de revistas | |