Nitric oxide-releasing poly(vinyl alcohol) film for increasing dermal vasodilation

dc.creatorMarcilli, RHM
dc.creatorde Oliveira, MG
dc.date2014
dc.dateAPR 1
dc.date2014-08-01T18:38:51Z
dc.date2015-11-26T17:02:15Z
dc.date2014-08-01T18:38:51Z
dc.date2015-11-26T17:02:15Z
dc.date.accessioned2018-03-28T23:50:20Z
dc.date.available2018-03-28T23:50:20Z
dc.identifierColloids And Surfaces B-biointerfaces. Elsevier Science Bv, v. 116, n. 643, n. 651, 2014.
dc.identifier0927-7765
dc.identifier1873-4367
dc.identifierWOS:000335706100086
dc.identifier10.1016/j.colsurfb.2013.10.036
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81800
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81800
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278874
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionPathological conditions associated with the impairment of nitric oxide (NO) production in the vasculature, such as Raynaud's syndrome and diabetic angiopathy, have stimulated the development of new biomaterials capable of delivering NO topically. With this purpose, we modified poly(vinyl-alcohol) (PVA) by chemically crosslinking it via esterification with mercaptosuccinic acid. This reaction allowed the casting of sulfhydrylated PVA (PVA-SH) films. Differential scanning calorimetry and X-ray diffractometry showed that the crosslinking reaction completely suppressed the crystallization of PVA, leading to a non-porous film with a homogeneous distribution of -SH groups. The remaining free hydroxyl groups in the PVA-SH network conferred partial hydrophylicity to the material, which was responsible for a swelling degree of ca. 110%. The PVA-SH films were subjected to an S-nitrosation reaction of the -SH groups, yielding a PVA containing S-nitrosothiol groups (PVA-SNO). Amperometric and chemiluminescence measurements showed that the PVA-SNO films were capable of releasing NO spontaneously after immersion in physiological medium. Laser Doppler-flowmetry, used to assess the blood flow in the dermal microcirculation, showed that the topical application of hydrated PVA-SNO films on the health skin led to a dose- and time-dependent increase of more than 5-fold in the dermal baseline blood flow in less than 10 min, with a prolonged action of more than 4 h during continuous application. These results show that PVA-SNO films might emerge as a new material with potential for the topical treatment of microvascular skin disorders. (C) 2013 Elsevier B.V. All rights reserved.
dc.description116
dc.description643
dc.description651
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCNPq [309390/2011-7]
dc.descriptionFAPESP [2008/57560-0]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationColloids And Surfaces B-biointerfaces
dc.relationColloid Surf. B-Biointerfaces
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectPoly(vinyl alcohol)
dc.subjectNitric oxide
dc.subjectMercaptosuccinic acid
dc.subjectTopical application
dc.subjectSkin vasodilation
dc.subjectMercaptosuccinic Acid
dc.subjectUrinary-excretion
dc.subjectChelating-agents
dc.subjectBlood-flow
dc.subjectHydrogels
dc.subjectChemiluminescence
dc.subjectTransport
dc.subjectPolymers
dc.subjectDelivery
dc.subjectBehavior
dc.titleNitric oxide-releasing poly(vinyl alcohol) film for increasing dermal vasodilation
dc.typeArtículos de revistas


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