Palatinose production by free and Ca-alginate gel immobilized cells of Erwinia sp.

dc.creatorKawaguti, HY
dc.creatorSato, HH
dc.date2007
dc.dateOCT 1
dc.date2014-11-19T04:05:18Z
dc.date2015-11-26T17:01:46Z
dc.date2014-11-19T04:05:18Z
dc.date2015-11-26T17:01:46Z
dc.date.accessioned2018-03-28T23:49:40Z
dc.date.available2018-03-28T23:49:40Z
dc.identifierBiochemical Engineering Journal. Elsevier Science Sa, v. 36, n. 3, n. 202, n. 208, 2007.
dc.identifier1369-703X
dc.identifierWOS:000250166500002
dc.identifier10.1016/j.bej.2007.02.017
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58994
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58994
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58994
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278783
dc.descriptionPalatinose is a non-cariogenic disaccharide obtained from the enzymatic conversion of sucrose, used in food industries as a sugar substitute. Free and Ca-alginate immobilized cells of Erwinia sp. D12 were used to produce palatinose from sucrose. Palatinose production was studied in a repeated-batch process using different immobilized biocatalysts: whole cells, disrupted cells and glucosyltransferase. Successive batches were treated with the immobilized biocatalyst, but a decrease in palatinose production was observed. A continuous process using a packed-bed reactor was investigated, and found to produce 55-66% of palatinose during 17 days using immobilized cells treated with glutaraldehyde and a substrate flow speed of 0.56 ml min(-1). However, immobilized cells in a packed-bed reactor failed to maintain the palatinose production for a prolonged period. The free cells showed a high conversion rate using batch fermentation, obtaining a palatinose yield of 77%. The cells remained viable for 16 cycles with high palatinose yields (65-77%). Free Erwinia sp. D 12 cells supported high production levels in repeated-batch operations, and the results showed the potential for repeated reuse. (C) 2007 Elsevier B.V. All rights reserved.
dc.description36
dc.description3
dc.description202
dc.description208
dc.languageen
dc.publisherElsevier Science Sa
dc.publisherLausanne
dc.publisherSuíça
dc.relationBiochemical Engineering Journal
dc.relationBiochem. Eng. J.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectbiocatalysis
dc.subjectfree-cells
dc.subjectimmobilized cells
dc.subjectpacked-bed bioreactor
dc.subjectpalatinose
dc.subjectrepeated-batch process
dc.subjectResponse-surface Methodology
dc.subjectSp D12 Cells
dc.subjectSerratia-plymuthica
dc.subjectIsomaltulose
dc.subjectSucrose
dc.subjectGlucosyltransferase
dc.subjectOptimization
dc.subjectRhapontici
dc.subjectConversion
dc.subjectBacterium
dc.titlePalatinose production by free and Ca-alginate gel immobilized cells of Erwinia sp.
dc.typeArtículos de revistas


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