Utilization of the etest assay for comparative antibiotic susceptibility profiles of citrus variegated chlorosis and Pierce's disease strains of Xylella fastidiosa

dc.creatorRibeiro, MD
dc.creatorDellias, MD
dc.creatorTsai, S
dc.creatorBolmstrom, A
dc.creatorMeinhardt, LW
dc.creatorBellato, CD
dc.date2005
dc.dateOCT
dc.date2014-11-19T03:22:12Z
dc.date2015-11-26T17:01:17Z
dc.date2014-11-19T03:22:12Z
dc.date2015-11-26T17:01:17Z
dc.date.accessioned2018-03-28T23:49:07Z
dc.date.available2018-03-28T23:49:07Z
dc.identifierCurrent Microbiology. Springer, v. 51, n. 4, n. 262, n. 266, 2005.
dc.identifier0343-8651
dc.identifierWOS:000232401200010
dc.identifier10.1007/s00284-005-0015-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73092
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73092
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73092
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278682
dc.descriptionXylella fastidiosa has a wide host range. Isolates of this bacterium that cause diseases in citrus (CVC) and grapes (PD) share 98% genome homology, and 95.7% amino acid identity. Drug resistance genes show a higher level of divergence and may be involved in the X. fastidiosa-host interaction. Antibiotic susceptibility of CVC and PD strains were compared utilizing the Etest strip method (AB Biodisk). Etest is applicable for fastidious slow-growing organisms due to its reproducibility. Results showed that the CVC strain was resistant to bacitracin, cefotaxime, and trimethoprim, and susceptible to chloramphenicol, erythromycin, gentamicin, kanamycin, streptomycin, and tetracycline. The PD strain was susceptible to all tested antibiotics, except kanamycin and trimethoprim. Both isolates produced a class C beta-lactamase. These data support previous antibiotic studies and gene discrepancies found in the sequencing data of PD and CVC strains. These results demonstrate the efficacy of utilizing Etest assays for X. fastidiosa strains.
dc.description51
dc.description4
dc.description262
dc.description266
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationCurrent Microbiology
dc.relationCurr. Microbiol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectOuter-membrane
dc.subjectBacteria
dc.subjectPathogen
dc.subjectBarrier
dc.titleUtilization of the etest assay for comparative antibiotic susceptibility profiles of citrus variegated chlorosis and Pierce's disease strains of Xylella fastidiosa
dc.typeArtículos de revistas


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