Effect of thiopental, pentobarbital and diethyl ether on early steps of insulin action in liver and muscle of the intact rat

dc.creatorCardoso, AR
dc.creatorCarvalho, CRO
dc.creatorVelloso, LA
dc.creatorBrenelli, SL
dc.creatorSaad, MJA
dc.creatorCarvalheira, JBC
dc.date2005
dc.dateAPR 1
dc.date2014-11-19T02:45:27Z
dc.date2015-11-26T17:01:00Z
dc.date2014-11-19T02:45:27Z
dc.date2015-11-26T17:01:00Z
dc.date.accessioned2018-03-28T23:48:48Z
dc.date.available2018-03-28T23:48:48Z
dc.identifierLife Sciences. Pergamon-elsevier Science Ltd, v. 76, n. 20, n. 2287, n. 2297, 2005.
dc.identifier0024-3205
dc.identifierWOS:000227580700002
dc.identifier10.1016/j.lfs.2004.10.043
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63980
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/63980
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63980
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278623
dc.descriptionA large number of experimental studies have investigated insulin signaling in rats. In these studies different anaesthetics have been used to anaesthetize rats. However, the direct effects of anaesthetics on the regulation of the early steps of insulin action are not known. In the present study, we investigated the effect of thiopental, pentobarbital and diethyl ether on the plasma glucose disappearance rate, IR, IRS-1 and IRS-2 tyrosine phosphorylation, IRSs association with PI 3-kinase, Akt and Erk phosphorylation, in liver and muscle of rats. Fasting plasma glucose levels were higher in animals anaesthetized with ether. No differences in plasma glucose disappearance rates were observed, however. Insulin-induced IR, IRS-1 and IRS-2 tyrosine phosphorylation, association of these substrates with PI 3-kinase and Akt and ERK phosphorylation were similar in the three groups of animals in both tissues. These data suggest that both thiopental and pentobarbital may be used in studies where changes in insulin signaling are being measured and where adequate general anaesthesia is required. (c) 2005 Elsevier Inc. All rights reserved.
dc.description76
dc.description20
dc.description2287
dc.description2297
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectpentobarbital
dc.subjectether
dc.subjectethyl
dc.subjectthiopental
dc.subjectsignal transduction
dc.subjectphosphoproteins
dc.subjectActivated Protein-kinase
dc.subjectGlycogen-synthase Kinase-3
dc.subjectPhosphatidylinositol 3-kinase
dc.subjectReceptor Substrate-1
dc.subjectSkeletal-muscle
dc.subjectCarbohydrate Metabolism
dc.subjectGlucose-transporter
dc.subjectBlood-pressure
dc.subjectDna-synthesis
dc.subjectHeart-rate
dc.titleEffect of thiopental, pentobarbital and diethyl ether on early steps of insulin action in liver and muscle of the intact rat
dc.typeArtículos de revistas


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