Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets

dc.creatorCrepaldiAlves, SC
dc.creatorCarneiro, EM
dc.creatorBosqueiro, JR
dc.creatorBoschero, AC
dc.date1997
dc.dateMAR
dc.date2014-12-16T11:33:37Z
dc.date2015-11-26T16:59:50Z
dc.date2014-12-16T11:33:37Z
dc.date2015-11-26T16:59:50Z
dc.date.accessioned2018-03-28T23:47:35Z
dc.date.available2018-03-28T23:47:35Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 30, n. 3, n. 359, n. 361, 1997.
dc.identifier0100-879X
dc.identifierWOS:A1997WQ16600008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74005
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/74005
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278312
dc.descriptionWe studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.3 mM glucose) was similar in control and PRL-treated islets (1.84 +/- 0.06% and 2.08 +/- 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 +/- 0.15% and 3.09 +/- 0.21% of the islet insulin content in control and 2.43 +/- 0.16% and 4.31 +/- 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 mu g/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.
dc.description30
dc.description3
dc.description359
dc.description361
dc.languageen
dc.publisherAssoc Bras Divulg Cientifica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Medical And Biological Research
dc.relationBrazilian J. Med. Biol. Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectpancreatic islets
dc.subjectglucose transporter
dc.subjectprolactin
dc.subjectglucose
dc.titleSynergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
dc.typeArtículos de revistas


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