Antioxidant defense and apoptotic effectors in ascorbic acid and beta-glycerophosphate-induced osteoblastic differentiation

dc.creatorNeto, AHC
dc.creatorMachado, D
dc.creatorYano, CL
dc.creatorFerreira, CV
dc.date2011
dc.dateJAN
dc.date2014-08-01T18:22:19Z
dc.date2015-11-26T16:59:37Z
dc.date2014-08-01T18:22:19Z
dc.date2015-11-26T16:59:37Z
dc.date.accessioned2018-03-28T23:47:17Z
dc.date.available2018-03-28T23:47:17Z
dc.identifierDevelopment Growth & Differentiation. Wiley-blackwell Publishing, Inc, v. 53, n. 1, n. 88, n. 96, 2011.
dc.identifier0012-1592
dc.identifierWOS:000286597800010
dc.identifier10.1111/j.1440-169X.2010.01232.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77912
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77912
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278239
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMC3T3-E1 cells grown in the presence of ascorbic acid and beta-glycerophosphate (AA/beta-GP) express alkaline phosphatase and produce an extensive collagenous extracellular matrix. Differentiated MC3T3-E1 cells are more sensitive to hydrogen peroxide-induced oxidative stress than undifferentiated cells. In this study, we compared the profile of antioxidant enzymes and molecular markers of apoptosis in undifferentiated and differentiated MC3T3-E1 cells (cell differentiation was induced by treatment with AA/beta-GP). Differentiated osteoblasts showed lower expression and activity of catalase, glutathione S-transferase and glutathione peroxidase. The total superoxide dismutase activity and the expression of Cu/Zn superoxide dismutase were also lower, while the expression of Mn superoxide dismutase was higher in differentiated osteoblasts. The level of malondialdehyde, a widely used marker for oxidative stress, was lower in the AA/beta-GP group compared with control cells, but this difference was not significant. Western blotting showed that treatment with AA/beta-GP increased the Bax/Bcl-2 ratio used as an index of cellular vulnerability to apoptosis. In addition, the activities of caspases 3, 8 and 9 and cleaved poly (ADP) ribose polymerase were significantly higher in differentiated cells. These findings provide new insights into how changes in the activities of major antioxidant enzymes and in the signaling pathways associated with apoptosis may influence the susceptibility of bone cells to oxidative stress.
dc.description53
dc.description1
dc.description88
dc.description96
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [06/00430-1]
dc.languageen
dc.publisherWiley-blackwell Publishing, Inc
dc.publisherMalden
dc.publisherEUA
dc.relationDevelopment Growth & Differentiation
dc.relationDev. Growth Diff.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectascorbic acid
dc.subjectbeta-glycerophosphate
dc.subjectdifferentiation
dc.subjectenzymes antioxidant
dc.subjectosteoblast
dc.subjectMaturation-dependent Vulnerability
dc.subjectMesenchymal Stem-cells
dc.subjectCyto-toxicity Assays
dc.subjectOxidative Stress
dc.subjectHydrogen-peroxide
dc.subjectMc3t3-e1 Cells
dc.subjectGlutathione-peroxidase
dc.subjectOsteogenic Differentiation
dc.subjectAlkaline-phosphatase
dc.subjectLipid-peroxidation
dc.titleAntioxidant defense and apoptotic effectors in ascorbic acid and beta-glycerophosphate-induced osteoblastic differentiation
dc.typeArtículos de revistas


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