Evaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticles

dc.creatorde Lima, R
dc.creatorPereira, ADS
dc.creatorPorto, RM
dc.creatorFraceto, LF
dc.date2011
dc.dateMAR
dc.date2014-07-30T17:27:57Z
dc.date2015-11-26T16:59:22Z
dc.date2014-07-30T17:27:57Z
dc.date2015-11-26T16:59:22Z
dc.date.accessioned2018-03-28T23:47:02Z
dc.date.available2018-03-28T23:47:02Z
dc.identifierJournal Of Polymers And The Environment. Springer/plenum Publishers, v. 19, n. 1, n. 196, n. 202, 2011.
dc.identifier1566-2543
dc.identifierWOS:000289801700025
dc.identifier10.1007/s10924-010-0262-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65793
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65793
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278171
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions containing three different concentrations of nanoparticles (5.4, 54 and 540 mu g/mL, polymer mass/volume of solution). The nanoparticles were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95 nm, a zeta potential of -20 mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations employed. The results provide new information concerning the toxic effects of particles produced using PLGA.
dc.description19
dc.description1
dc.description196
dc.description202
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherSpringer/plenum Publishers
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Polymers And The Environment
dc.relationJ. Polym. Environ.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectPolymeric nanoparticles
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectEnvironmental toxicity
dc.subjectIn-vitro
dc.subjectCytotoxicity
dc.subjectParticles
dc.subjectToxicity
dc.subjectToxicology
dc.subjectCells
dc.subjectDrug
dc.subjectNanotechnology
dc.subjectSystems
dc.subjectCarrier
dc.titleEvaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticles
dc.typeArtículos de revistas


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