Artículos de revistas
Purification of n-boc-Rolipram racemate on chiral stationary phase using simulated moving bed chromatography under linear conditions
Registro en:
Biochemical Engineering Journal. Elsevier Science Sa, v. 40, n. 3, n. 526, n. 536, 2008.
1369-703X
WOS:000257536600018
10.1016/j.bej.2008.02.008
Autor
Goncalves, CV
Carpes, MJS
Correia, CRD
Santana, CC
Institución
Resumen
Chiral stationary phases (CSPs) associated with simulated moving bed (SMB) technology have shown good performance in the purification of racemates. Rolipram, (+/-)-4-(3-(cyclopentyloxy)-4-methoxyphenyl)-pyrrolidin-2-one), is an antidepressant with significant pharmacological properties. The aim of this work was to purify the n-boc-Rolipram racemate (Rolipram intermediate) on cellulose tris(3,5-dimethylphenylcarbamate) CSP coated on silica by simulated moving bed (SMB) continuous chromatography. The experiments were carried out under diluted conditions. Henry's constants were determined and the system revealed selectivity close to 1.26. Purity and enantiomeric excess of the extract and raffinate streams were evaluated and discussed. Performance parameters of the studied system were determined also. Results showed reasonable performance of the SMB unit to separate the n-boc-Rolipram enantiomers. (c) 2008 Elsevier B.V. All rights reserved. 40 3 526 536