A new experimental model for inducing interstitial cystitis by oxidative stress using bladder instillation of a nitric oxide donor gel

dc.creatorPalma, TF
dc.creatorSeabra, A
dc.creatorSouto, SC
dc.creatorMaciel, L
dc.creatorAlvarenga, M
dc.creatorSiniscalchi, R
dc.creatorGanzarolli, M
dc.creatorRicetto, C
dc.date2011
dc.dateMAY
dc.date2014-08-01T18:15:42Z
dc.date2015-11-26T16:59:14Z
dc.date2014-08-01T18:15:42Z
dc.date2015-11-26T16:59:14Z
dc.date.accessioned2018-03-28T23:46:54Z
dc.date.available2018-03-28T23:46:54Z
dc.identifierActas Urologicas Espanolas. Ene Ediciones Sl, v. 35, n. 5, n. 253, n. 258, 2011.
dc.identifier0210-4806
dc.identifierWOS:000290512200001
dc.identifier10.1016/j.acuro.2010.05.018
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76178
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76178
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278141
dc.descriptionPurpose: The aim of this study is to develop a new experimental model of inducing interstitial cystitis (IC) through vesical instillation of a polymeric solution containing the NO donor S-nitrousglutathione (GSNO) and to compare it to the experimental interstitial cystitis induced by vesical instillation of protamine and potassium chloride. Material and method: For that purpose 40 female Wistar rats were used, divided in four groups: 1. saline solution + GSNO; 2. saline solution + polymeric solution (without GNSO); 3. protamine sulphate + KCl; 4. protamine sulphate + GSNO. The rats received one application (5 animals) or 3 applications (5 animals) of the corresponding substance through intravesical instillation, and after 6 days (5 animals) or 9 days (5 animals) they were euthanized and their bladders were removed for macroscopic evaluation and histological study. Results: In the macroscopic evaluation we observed edema and hyperemia of the mucosa in 2 (22%) of the animals in group 1, in 0 (0%) of the animals in group 2, in 10 (100%) of the animals in group 3, and in 5 (50%) of the animals in group 4. In the protamine + KCl group and in saline + GSNO similar effects were observed on the bladder wall. The animals in group 2 (saline + polymeric) showed vascular congestion, significantly smaller than the rest after 9 days instillations (p = 0.0035). Significant increased fibrosis was observed after instillations in groups 3 and 4, after 6 days (p = 0.3781) and 9 days (p = 0.0459) respectively, when compared to control (group 2). All groups presented neutrophilic infiltrate of variable intensity 6 days after instillations (p = 0.7277). After 9 days, there was a regression of the infiltrate, with no evidence of accentuated neutrophilic reaction in all the groups (p = 0.2301). Conclusion: The inflammatory response to bladder instillation of an aqueous solution of S-nitrousglutathione was very similar to that induced by bladder instillation of protamine and KCl. Instillation of an aqueous solution of GSNO can be considered a new model for experimental induction of interstitial cystitis. (C) 2010 AEU. Published by Elsevier Espana, S.L. All rights reserved.
dc.description35
dc.description5
dc.description253
dc.description258
dc.languagees
dc.publisherEne Ediciones Sl
dc.publisherMadrid
dc.publisherEspanha
dc.relationActas Urologicas Espanolas
dc.relationActas Urol. Esp.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectInterstitial Cystitis
dc.subjectNitric Oxide
dc.subjectIn vivo model
dc.subjectExpression
dc.subjectSynthase
dc.titleA new experimental model for inducing interstitial cystitis by oxidative stress using bladder instillation of a nitric oxide donor gel
dc.typeArtículos de revistas


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