A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T

dc.creatorFonseca, RAS
dc.creatorRamos-Jesus, J
dc.creatorKubota, LT
dc.creatorDutra, RF
dc.date2011
dc.dateNOV
dc.date2014-08-01T18:15:37Z
dc.date2015-11-26T16:58:52Z
dc.date2014-08-01T18:15:37Z
dc.date2015-11-26T16:58:52Z
dc.date.accessioned2018-03-28T23:46:30Z
dc.date.available2018-03-28T23:46:30Z
dc.identifierSensors. Mdpi Ag, v. 11, n. 11, n. 10785, n. 10797, 2011.
dc.identifier1424-8220
dc.identifierWOS:000297698600046
dc.identifier10.3390/s111110785
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76130
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76130
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1278044
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionA piezoelectric immunosensor based on gold nanoparticles (AuNPs) co-immobilized on a dithiol-modified surface is proposed for detection of human cardiac troponin T (TnT). Anti-human troponin T (anti-TnT) antibodies were covalently immobilized on the nanostructured electrode surface by thiol-aldehyde linkages. In a homogeneous bulk solution, TnT was captured by anti-TnT immobilized on the QCM electrode. Cyclic voltammetry studies were used to characterize the AuNPs layer on the electrode surface and the anti-TnT immobilization steps. The QCM-flow immunosensor exhibited good reliability, measuring concentrations of TnT from 0.003 to 0.5 ng mL(-1) in human serum with high linearity (r = 0.989; p < 0.01). The immunosensor exhibited a 7% coefficient of variation and 0.0015 ng mL(-1) limit of detection, indicating a high reproducibility and sensitivity. The proposed QCM nanostructured immunosensor is easy to use and has promising potential in the diagnosis of acute myocardial infarction due to its speed and high sensitivity.
dc.description11
dc.description11
dc.description10785
dc.description10797
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [483242/2010-1]
dc.languageen
dc.publisherMdpi Ag
dc.publisherBasel
dc.publisherSuíça
dc.relationSensors
dc.relationSensors
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectpiezoelectric
dc.subjectimmunosensor
dc.subjectgold nanoparticle
dc.subjectcardiac troponin T
dc.subjectFlow-injection Immunoassay
dc.subjectElectrochemical Biosensors
dc.subjectGold
dc.subjectNanoparticles
dc.subjectSurface
dc.subjectAmplification
dc.subjectElectrode
dc.subjectAntigen
dc.subjectMarkers
dc.titleA Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T
dc.typeArtículos de revistas


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