dc.creator | Machado, SW | |
dc.creator | de Oliveira, CFR | |
dc.creator | Bezerra, CDS | |
dc.creator | Freire, MDM | |
dc.creator | Kill, MR | |
dc.creator | Machado, OLT | |
dc.creator | Marangoni, S | |
dc.creator | Macedo, MLR | |
dc.date | 2013 | |
dc.date | 41334 | |
dc.date | 2014-07-30T14:18:43Z | |
dc.date | 2015-11-26T16:58:51Z | |
dc.date | 2014-07-30T14:18:43Z | |
dc.date | 2015-11-26T16:58:51Z | |
dc.date.accessioned | 2018-03-28T23:46:29Z | |
dc.date.available | 2018-03-28T23:46:29Z | |
dc.identifier | Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 61, n. 10, n. 2469, n. 2478, 2013. | |
dc.identifier | 0021-8561 | |
dc.identifier | WOS:000316243900022 | |
dc.identifier | 10.1021/jf3049565 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58472 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/58472 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1278039 | |
dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description | Anagasta kuehniella is a polyphagous pest that causes economic losses worldwide. This species produces serine proteases as its major enzymes for protein digestion. In this study, a new serine-protease inhibitor was isolated from Acacia polyphylla seeds (AcKI). Further analysis revealed that AcKI is formed by two polypeptide chains with a relative molecular mass of similar to 20 kDa. The effects of AcKI on the development, survival, and enzymatic activity of Anagasta kuehniella larvae were evaluated, by incorporating AcKI in an artificial diet Bioassays revealed a reduction in larval weight of similar to 50% with the lower concentration of AcKI used in the study (0.5%). Although additional assays showed an increase in endogenous trypsin and chymotrypsin activities, with a degree of AcKI-insensivity, AcKI produces an anti nutritional effect on A. kuehniella, indicating AcKI as a promising bioinsecticide protein for engineering plants that are resistant to insect pests. | |
dc.description | 61 | |
dc.description | 10 | |
dc.description | 2469 | |
dc.description | 2478 | |
dc.description | FINEP | |
dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description | FUNDECT | |
dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.language | en | |
dc.publisher | Amer Chemical Soc | |
dc.publisher | Washington | |
dc.publisher | EUA | |
dc.relation | Journal Of Agricultural And Food Chemistry | |
dc.relation | J. Agric. Food Chem. | |
dc.rights | fechado | |
dc.source | Web of Science | |
dc.subject | Acacia polyphylla | |
dc.subject | Kunitz-type inhibitor | |
dc.subject | Anagasta kuehniella | |
dc.subject | pest insect | |
dc.subject | Serine Proteinase-inhibitors | |
dc.subject | Trypsin-inhibitor | |
dc.subject | Larval Development | |
dc.subject | Digestive Enzymes | |
dc.subject | Bioinsecticidal Activity | |
dc.subject | Hubner Lepidoptera | |
dc.subject | Helicoverpa-zea | |
dc.subject | Chymotrypsin | |
dc.subject | Midgut | |
dc.subject | Growth | |
dc.title | Purification of a Kunitz-type Inhibitor from Acacia polyphylla DC Seeds: Characterization and Insecticidal Properties against Anagasta kuehniella Zeller (Lepidoptera: Pyralidae) | |
dc.type | Artículos de revistas | |