STREPTOZOTOCIN-INDUCED TOXICITY IN CHO-9 AND V79 CELLS

dc.creatorCAPUCCI, MS
dc.creatorHOFFMANN, ME
dc.creatorDEGROOT, A
dc.creatorNATARAJAN, AT
dc.date1995
dc.date2014-12-16T11:33:23Z
dc.date2015-11-26T16:58:40Z
dc.date2014-12-16T11:33:23Z
dc.date2015-11-26T16:58:40Z
dc.date.accessioned2018-03-28T23:46:18Z
dc.date.available2018-03-28T23:46:18Z
dc.identifierEnvironmental And Molecular Mutagenesis. Wiley-liss, v. 26, n. 1, n. 72, n. 78, 1995.
dc.identifier0893-6692
dc.identifierWOS:A1995RM10400010
dc.identifier10.1002/em.2850260111
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/75173
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/75173
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/75173
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277987
dc.descriptionThe cytotoxicity of streptozotocin (STZ) was investigated in Chinese hamster fibroblast lines (CHO-9 and V79) in comparison to two other alkylating agents, methylnitrosourea (MNU) and ethylnitrosourea (ENU), using cell survival as the endpoint. it was found that V79 cells were felt more resistant to methylation induced by STZ and MNU than CHO-9 cells (20 and four times, respectively) but equally sensitive to the ethylating agent ENU. The extent of STZ-induced DNA methylation was estimated by analyzing the extent of O-6-metG and N7-metG adducts in the DNA of treated cells through high-performance liquid chromatography (HPLC) with electrochemical detection. The number of adducts as well the efficiencies of their removal from the DNA were similar in both cell lines. The response of these cells to the presence of DNA damage was evaluated by analysis of STZ effects on DNA replication and cell cycle progression. Measurement of [H-3]-thymidine incorporation showed a similar pattern of response at the level of inhibition of DNA synthesis in both cell lines. However, analysis of meta-phase cells 36 hr after STZ exposure showed on accumulation of cells in the second cycle in the CHO-9 line, indicating induction of a cell cycle arrest. Only a slight effect was observed on cell cycle progression in V79 cells, indicating that the methylation resistance of these cells could be related to their ability to progress through the cell cycle despite the presence of DNA lesions. (C) 1995 Wiley-Liss, Inc.
dc.description26
dc.description1
dc.description72
dc.description78
dc.languageen
dc.publisherWiley-liss
dc.publisherNew York
dc.relationEnvironmental And Molecular Mutagenesis
dc.relationEnviron. Mol. Mutagen.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectSTREPTOZOTOCIN
dc.subjectMAMMALIAN CELLS
dc.subjectDNA METHYLATION
dc.subjectCELL CYCLE
dc.subjectSister Chromatid Exchanges
dc.subjectChinese-hamster Cells
dc.subjectDna Damage
dc.subjectMammalian-cells
dc.subjectRepair
dc.subjectMethylnitrosourea
dc.subject6-thioguanine
dc.subjectSensitivity
dc.subjectResistance
dc.subjectAlkylation
dc.titleSTREPTOZOTOCIN-INDUCED TOXICITY IN CHO-9 AND V79 CELLS
dc.typeArtículos de revistas


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