Measuring cytoplasmic calcium level in Citrus protoplasts using the fluorescent probe indo-1

dc.creatorGouvea, CMCP
dc.creatorVidal, BC
dc.creatorMartins, IS
dc.date1997
dc.dateSEP
dc.date2014-12-16T11:33:19Z
dc.date2015-11-26T16:58:31Z
dc.date2014-12-16T11:33:19Z
dc.date2015-11-26T16:58:31Z
dc.date.accessioned2018-03-28T23:46:07Z
dc.date.available2018-03-28T23:46:07Z
dc.identifierJournal Of Plant Physiology. Gustav Fischer Verlag, v. 151, n. 3, n. 329, n. 333, 1997.
dc.identifier0176-1617
dc.identifierWOS:A1997XY68500011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53456
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/53456
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53456
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277942
dc.descriptionWe measured cytoplasmic free calcium in protoplasts of Citrus sinensis Osb. Protoplasts were isolated from cell-suspension cultures grown as microcalli. The cell surface presented acid polysaccharide filaments, probably responsible for cell adhesion. These filaments were disrupted by enzymatic digestion liberating the cells and subsequently the protoplasts. Protoplasts were loaded with the pentapotassium salt of indo-1 by incubation in acidic solutions of this calcium indicator. Loading increased with decreasing pH and osmotica. Dye loaded in this manner was distributed only in the cytoplasm. The protoplast autofluorescence was very low and did not interfere with measurements. Cytoplasmic free-calcium concentration in Citrus protoplasts, using microspectrofluorimetry, was estimated to be 154 nmol.L-1. This resting value was increased when protoplasts were exposed to high extracellular Ca2+ (1 mmol.L-1) or 6BA (1 mu mol.L-1) suggesting that the pathway in cytokinin signal transduction in these cells may involve a Ca2+ uptake facilitation from extracellular medium.
dc.description151
dc.description3
dc.description329
dc.description333
dc.languageen
dc.publisherGustav Fischer Verlag
dc.publisherJena
dc.publisherAlemanha
dc.relationJournal Of Plant Physiology
dc.relationJ. Plant Physiol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectCitrus sinensis Osb.
dc.subjectcytokinin
dc.subjectcytosolic calcium
dc.subjectindo-1
dc.subjectprotoplast
dc.subjectSignal-transduction
dc.subjectCytosolic Calcium
dc.subjectAleurone Protoplasts
dc.subjectPlant-cells
dc.subjectCytokinin
dc.subjectRegeneration
dc.subjectCulture
dc.subjectCa-2+
dc.subjectCa2+
dc.titleMeasuring cytoplasmic calcium level in Citrus protoplasts using the fluorescent probe indo-1
dc.typeArtículos de revistas


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