Cytoskeletal rearrangement and cell death induced by Bothrops alternatus snake venom in cultured Madin-Darby canine kidney cells

dc.creatorNascimento, JM
dc.creatorFranchi, GC
dc.creatorNowill, AE
dc.creatorCollares-Buzato, CB
dc.creatorHyslop, S
dc.date2007
dc.dateOCT
dc.date2014-11-18T18:24:41Z
dc.date2015-11-26T16:57:49Z
dc.date2014-11-18T18:24:41Z
dc.date2015-11-26T16:57:49Z
dc.date.accessioned2018-03-28T23:45:25Z
dc.date.available2018-03-28T23:45:25Z
dc.identifierBiochemistry And Cell Biology-biochimie Et Biologie Cellulaire. Natl Research Council Canada-n R C Research Press, v. 85, n. 5, n. 591, n. 605, 2007.
dc.identifier0829-8211
dc.identifierWOS:000250624500007
dc.identifier10.1139/O07-067
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57615
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57615
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57615
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277771
dc.descriptionBothrops snake venoms cause renal damage, with renal failure being the main cause of death in humans bitten by these snakes. In this work, we investigated the cytoskeletal rearrangement and cytotoxicity caused by Bothrops alternatus venom in cultured Madin-Darby canine kidney (MDCK) cells. Incubation with venom (10 and 100 mu g/mL) significantly (p <0.05) decreased the cellular uptake of neutral red dye after 1 and 3 h. Venom (100 mu g/mL) also markedly decreased the transepithelial electrical resistance (R-T) across MDCK monolayers. Staining with rhodamine-conjugated phalloidin revealed disarray of the cytoskeleton that involved the stress fibers at the basal cell surface and focal adhesion-associated F-actin in the cell-matrix contact region. Feulgen staining showed a significant decrease in the number of cells undergoing mitosis and an increase in the frequency of altered nuclei. Scanning electron microscopy revealed a decrease in the number of microvilli and the presence of cells with a fusiform format. Flow cytometry with annexin V and propidium iodide showed that cell death occurred by necrosis, with little apoptosis, a conclusion supported by the lack of DNA fragmentation characteristic of apoptosis. Pretreating the cells with catalase significantly attenuated the venom-induced loss of viability, indicating a possible involvement of H2O2 in the cellular damage; less protection was observed with superoxide dismutase or N-w-nitro-L-arginine methyl ester. These results indicate that Bothrops alternatus venom is cytotoxic to cultured MDCK cells, possibly via the action of reactive oxygen species. This cytotoxicity could contribute to nephrotoxicity after envenoming by this species.
dc.description85
dc.description5
dc.description591
dc.description605
dc.languageen
dc.publisherNatl Research Council Canada-n R C Research Press
dc.publisherOttawa
dc.publisherCanadá
dc.relationBiochemistry And Cell Biology-biochimie Et Biologie Cellulaire
dc.relationBiochem. Cell Biol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectapoptosis
dc.subjectBothrops alternatus
dc.subjectcytotoxicity
dc.subjectMDCK cells
dc.subjectnecrosis
dc.subjectrenal failure
dc.subjectDisintegrin-like Protein
dc.subjectAmino-acid Oxidase
dc.subjectLance-headed Viper
dc.subjectPhospholipase A(2)
dc.subjectIn-vitro
dc.subjectJararaca Venom
dc.subjectParacellular Barrier
dc.subjectNeutrophil Migration
dc.subjectActin Cytoskeleton
dc.subjectEndothelial-cells
dc.titleCytoskeletal rearrangement and cell death induced by Bothrops alternatus snake venom in cultured Madin-Darby canine kidney cells
dc.typeArtículos de revistas


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