Modulation of gap and adherens junctional proteins in cultured neonatal pancreatic islets

dc.creatorCollares-Buzato, CB
dc.creatorLeite, AR
dc.creatorBoschero, AC
dc.date2001
dc.dateAUG
dc.date2014-11-18T17:08:00Z
dc.date2015-11-26T16:57:24Z
dc.date2014-11-18T17:08:00Z
dc.date2015-11-26T16:57:24Z
dc.date.accessioned2018-03-28T23:44:56Z
dc.date.available2018-03-28T23:44:56Z
dc.identifierPancreas. Lippincott Williams & Wilkins, v. 23, n. 2, n. 177, n. 185, 2001.
dc.identifier0885-3177
dc.identifierWOS:000170027900008
dc.identifier10.1097/00006676-200108000-00008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57687
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57687
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57687
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277650
dc.descriptionFetal and neonatal pancreatic islets have lower insulin secretory responses compared with adult islets. In culture conditions and after treatment with mammosomatotropic hormones, neonatal islets undergo maturation of the secretory machinery that might involve regulation of cell-cell contacts within the islet. This study is an investigation of the effect of prolonged culturing and in vitro treatment with prolactin on the expression of the gap junction-associated connexin 43 and the adherens junction-associated beta -catenin in cultured neonatal rat islets. Pancreatic islets from neonatal Wistar rats were cultured for 24 hours or 7 days, and the treated group was exposed to 2 mug/mL prolnctin daily for 7 days. Connexin 43 and beta -catenin were barely detected at the cell-cell contacts in 24-hour-cultured islets, as revealed by immunocytochemical analysis. Nevertheless, both junctional proteins were well expressed at the junctional region in islet cells cultured for 7 days and showed even greater staining in islets after long-term prolactin treatment. In accordance with the morphologic data, neonatal islets cultured for 24 hours displayed a relatively low level of connexin 43, as determined by Western blot analysis. Culturing for 7 days or combined prolactin treatment induced a significant increase in connexin 43 expression; this was 40% greater in the prolactin-treated group than in the control group. Furthermore, an enhancement of the expression of beta -catenin and translocation of this protein to the cell-cell contact site was also observed in neonatal islets cultured for 7 days compared with those cultured for 24 hours. In vitro prolactin treatment induced even greater expression of beta -catenin in islet cells. A correlation was observed between the increased expression of these junctional proteins and an increase in insulin secretion in cultured neonatal islets. In conclusion, prolonged culturing and in vitro treatment with prolactin induce the modulation of gap and adherens junctional proteins in pancreatic islets, which may be an important event in the in vitro maturation process of neonatal islet cells.
dc.description23
dc.description2
dc.description177
dc.description185
dc.languageen
dc.publisherLippincott Williams & Wilkins
dc.publisherPhiladelphia
dc.publisherEUA
dc.relationPancreas
dc.relationPancreas
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcell-cell contact
dc.subjectconnexin 43
dc.subjectbeta-catenin
dc.subjectislets of Langerhans
dc.subjectprolactin
dc.subjectislet culture
dc.subjectFetal-rat Islets
dc.subjectB-cells
dc.subjectInsulin-secretion
dc.subjectE-cadherin
dc.subjectIntercellular Communication
dc.subjectSignal-transduction
dc.subjectInvivo Modulation
dc.subjectGrowth-hormone
dc.subjectBeta-catenin
dc.subjectProlactin
dc.titleModulation of gap and adherens junctional proteins in cultured neonatal pancreatic islets
dc.typeArtículos de revistas


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