Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds

dc.creatorChiste, RC
dc.creatorYamashita, F
dc.creatorGozzo, FC
dc.creatorMercadante, AZ
dc.date2011
dc.date39083
dc.date2014-07-30T19:57:51Z
dc.date2015-11-26T16:57:08Z
dc.date2014-07-30T19:57:51Z
dc.date2015-11-26T16:57:08Z
dc.date.accessioned2018-03-28T23:44:39Z
dc.date.available2018-03-28T23:44:39Z
dc.identifierJournal Of Chromatography A. Elsevier Science Bv, v. 1218, n. 1, n. 57, n. 63, 2011.
dc.identifier0021-9673
dc.identifier1873-3778
dc.identifierWOS:000286494100007
dc.identifier10.1016/j.chroma.2010.10.094
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74222
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74222
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277578
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThis study was designed to identify and quantify the carotenoids and phenolic compounds from annatto seeds using high performance liquid chromatography coupled to diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). Furthermore, using response surface methodology, an optimized procedure for simultaneous extraction of these compounds was established. In addition to bixin, known to be the main carotenoid in annatto seeds, hypolaetin and a caffeoyl acid derivative were identified as the main phenolic compounds. The optimized procedure involved 15 extractions using acetone:methanol:water (50:40:10, v/v/v) as solvent, a solid-liquid ratio of 1:9 (m/v) and an extraction time of 5 min. Validation data indicated that the HPLC method proposed provided good linearity, sensitivity, procedure accuracy, system precision and suggested its suitability for the simultaneous analysis of phenolic compounds and carotenoids in annatto seeds. (C) 2010 Elsevier B.V. All rights reserved.
dc.description1218
dc.description1
dc.description57
dc.description63
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography A
dc.relationJ. Chromatogr. A
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBixa orellana L.
dc.subjectCarotenoids
dc.subjectPhenolic compounds
dc.subjectResponse surface methodology
dc.subjectHPLC-DAD-MS/MS
dc.subjectOrellana Seeds
dc.subjectAntioxidant
dc.subjectOptimization
dc.subjectChemistry
dc.subjectLycopene
dc.subjectModel
dc.subjectAcid
dc.subjectMs
dc.titleSimultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds
dc.typeArtículos de revistas


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