Pulsed LLLT improves tendon healing in rats: a biochemical, organizational, and functional evaluation

dc.creatorGuerra, FD
dc.creatorVieira, CP
dc.creatorde Almeida, MD
dc.creatorOliveira, LP
dc.creatorClaro, ACF
dc.creatorSimoes, GF
dc.creatorde Oliveira, ALR
dc.creatorPimentel, ER
dc.date2014
dc.dateMAR
dc.date2014-07-30T14:18:38Z
dc.date2015-11-26T16:56:54Z
dc.date2014-07-30T14:18:38Z
dc.date2015-11-26T16:56:54Z
dc.date.accessioned2018-03-28T23:44:24Z
dc.date.available2018-03-28T23:44:24Z
dc.identifierLasers In Medical Science. Springer London Ltd, v. 29, n. 2, n. 805, n. 811, 2014.
dc.identifier0268-8921
dc.identifier1435-604X
dc.identifierWOS:000333051700052
dc.identifier10.1007/s10103-013-1406-2
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58430
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58430
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277518
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn the last decades, the tendon injuries have increased substantially. Previous results suggested that low-level laser treatment (LLLT) promotes synthesis of extracellular matrix and improves the functional properties of the tendon. The aim of this study was to evaluate the effects of different protocols of LLLT on partially tenotomized tendons. Adult male rats were divided into the following: G1-intact, G2-injured, G3-injured + LLLT (4 J/cm(2) continuous), G4-injured + LLLT (4 J/cm(2) at 20 Hz). G2, G3, and G4 were euthanized 8 days after injury. G5-injured, G6-injured + LLLT (4 J/cm(2) continuous), and G7-injured + LLL (4 J/cm(2) at 20 Hz until the seventh day and 2 kHz from 8 to 14 days). G5, G6, and G7 were euthanized on the 15th day. Glycosaminoglycan (GAG) level was quantified by dimethylmethylene blue method and analyzed on agarose gel. Toluidine blue (TB) stain was used to observe metachromasy. CatWalk system was used to evaluate gait recovery. Collagen organization was analyzed by polarization microscopy. The GAG level increased in all transected groups, except G5. In G6 and G7, there was a significant increase in GAG in relation to G5. In G3 and G4, the presence of dermatan sulfate band was more prominent than G2. TB stains showed intense metachromasy in the treated groups. Birefringence analysis showed improvement in collagen organization in G7. The gait was significantly improved in G7. In conclusion, pulsed LLLT leads to increased organization of collagen bundles and improved gait recovery.
dc.description29
dc.description2
dc.descriptionSI
dc.description805
dc.description811
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2010/52383-2]
dc.languageen
dc.publisherSpringer London Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationLasers In Medical Science
dc.relationLasers Med. Sci.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectAchilles tendon
dc.subjectGait analysis
dc.subjectRehabilitation program
dc.subjectSoft tissue injuries
dc.subjectCollagen
dc.subjectHuman Achilles-tendon
dc.subjectLaser Irradiation
dc.subjectCollagens
dc.subjectInjury
dc.subjectGlycosaminoglycans
dc.subjectTendinopathy
dc.subjectBehavior
dc.subjectRuptures
dc.subjectRepair
dc.titlePulsed LLLT improves tendon healing in rats: a biochemical, organizational, and functional evaluation
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución