Simultaneous determination of tetracyclines in pharmaceuticals by CZE using experimental design

dc.creatorMamani, MCV
dc.creatorFarfan, JA
dc.creatorReyes, FGR
dc.creatorRath, S
dc.date2006
dc.dateSEP 15
dc.date2014-11-18T14:35:01Z
dc.date2015-11-26T16:55:56Z
dc.date2014-11-18T14:35:01Z
dc.date2015-11-26T16:55:56Z
dc.date.accessioned2018-03-28T23:43:24Z
dc.date.available2018-03-28T23:43:24Z
dc.identifierTalanta. Elsevier Science Bv, v. 70, n. 2, n. 236, n. 243, 2006.
dc.identifier0039-9140
dc.identifierWOS:000240418700002
dc.identifier10.1016/j.talanta.2006.02.048
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74215
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/74215
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74215
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277258
dc.descriptionAn optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DXC) is described. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated. A factorial and central composite design was performed to optimize the method in a simple way. The optimal separation conditions were 50 mmol L-1 sodium carbonate + 1 mmol L-1 EDTA, pH 10; voltage 13 kV and temperature 23 degrees C. The method was validated for TC determination in pharmaceuticals through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-assay and inter-assay precision, detectability, accuracy and ruggedness. In comparison with the recommended HPLC method in the United States Pharmacopeia, this CZE-method exhibited the same performance as the official method, with the advantage that the same method could be used for the simultaneous determination of the different tetracyclines in pharmaceutical formulations. (c) 2006 Elsevier B.V. All rights reserved.
dc.description70
dc.description2
dc.description236
dc.description243
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationTalanta
dc.relationTalanta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectantibiotics
dc.subjecttetracyclines
dc.subjectcapillary electrophoresis
dc.subjectvalidation
dc.subjectCapillary-electrophoresis
dc.subjectAntibiotics
dc.subjectOxytetracycline
dc.subjectSeparation
dc.titleSimultaneous determination of tetracyclines in pharmaceuticals by CZE using experimental design
dc.typeArtículos de revistas


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