Western diet modulates insulin signaling, c-jun N-terminal kinase activity, and insulin receptor substrate-1(ser307) phosphorylation in a tissue-specific fashion

dc.creatorPrada, PO
dc.creatorZecchin, HG
dc.creatorGasparetti, AL
dc.creatorTorsoni, MA
dc.creatorUeno, M
dc.creatorHirata, AE
dc.creatordo Amaral, MEC
dc.creatorHoer, NF
dc.creatorBoschero, AC
dc.creatorSaad, MJA
dc.date2005
dc.dateMAR
dc.date2014-07-30T19:02:48Z
dc.date2015-11-26T16:55:24Z
dc.date2014-07-30T19:02:48Z
dc.date2015-11-26T16:55:24Z
dc.date.accessioned2018-03-28T23:42:42Z
dc.date.available2018-03-28T23:42:42Z
dc.identifierEndocrinology. Endocrine Soc, v. 146, n. 3, n. 1576, n. 1587, 2005.
dc.identifier0013-7227
dc.identifierWOS:000227035400070
dc.identifier10.1210/en.2004-0767
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72837
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72837
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277094
dc.descriptionThe mechanisms by which diet-induced obesity is associated with insulin resistance are not well established, and no study has until now integrated, in a temporal manner, functional insulin action data with insulin signaling in key insulin-sensitive tissues, including the hypothalamus. In this study, we evaluated the regulation of insulin sensitivity by hyperinsulinemic-euglycemic clamp procedures and insulin signaling, c-jun N-terminal kinase (JNK) activation and insulin receptor substrate (IRS)-1(ser307) phosphorylation in liver, muscle, adipose tissue, and hypothalamus, by immunoprecipitation and immunoblotting, in rats fed on a Western diet (WD) or control diet for 10 or 30 d. WD increased visceral adiposity, serum triacylglycerol, and insulin levels and reduced whole-body glucose use. After 10 d of WD (WD10) there was a decrease in IRS-1/phosphatidylinositol 3-kinase/protein kinase B pathway in hypothalamus and muscle, associated with an attenuation of the anorexigenic effect of insulin in the former and reduced glucose transport in the latter. In WD10, there was an increased glucose transport in adipose tissue in parallel to increased insulin signaling in this tissue. After 30 d of WD, insulin was less effective in suppressing hepatic glucose production, and this was associated with a decrease in insulin signaling in the liver. JNK activity and IRS-1(ser307) phosphorylation were higher in insulin-resistant tissues. In summary, the insulin resistance induced by WD is tissue specific and installs first in hypothalamus and muscle and later in liver, accompanied by activation of JNK and IRS-1(ser307) phosphorylation. The impairment of the insulin signaling in these tissues, but not in adipose tissue, may lead to increased adiposity and insulin resistance in the WD rats.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description146
dc.description3
dc.description1576
dc.description1587
dc.languageen
dc.publisherEndocrine Soc
dc.publisherChevy Chase
dc.publisherEUA
dc.relationEndocrinology
dc.relationEndocrinology
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectObese Zucker Rats
dc.subjectPhosphatidylinositol 3-kinase
dc.subjectTranscription Factors
dc.subjectInduced Anorexia
dc.subjectArcuate Nucleus
dc.subjectAdipose-tissue
dc.subjectBody-weight
dc.subjectResistance
dc.subjectMice
dc.subjectMuscle
dc.titleWestern diet modulates insulin signaling, c-jun N-terminal kinase activity, and insulin receptor substrate-1(ser307) phosphorylation in a tissue-specific fashion
dc.typeArtículos de revistas


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