Electrochemical investigations of the reaction mechanism and kinetics between NADH and redox-active (NC)(2)C6H3-NHOH/(NC)(2)C6H3-NO from 4-nitrophthalonitrile-(NC)(2)C6H3-NO2-modified electrode

dc.creatorLima, PR
dc.creatorSantos, WDR
dc.creatorde Oliveira, AB
dc.creatorGoulart, MOF
dc.creatorKubota, LT
dc.date2008
dc.date42309
dc.date2014-11-18T12:40:40Z
dc.date2015-11-26T16:54:53Z
dc.date2014-11-18T12:40:40Z
dc.date2015-11-26T16:54:53Z
dc.date.accessioned2018-03-28T23:42:12Z
dc.date.available2018-03-28T23:42:12Z
dc.identifierBiosensors & Bioelectronics. Elsevier Advanced Technology, v. 24, n. 3, n. 448, n. 454, 2008.
dc.identifier0956-5663
dc.identifierWOS:000259834300017
dc.identifier10.1016/j.bios.2008.04.023
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65174
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65174
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65174
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276977
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionA simple and sensitive method for the electrocatalytic detection of NADH on a carbon paste electrode modified with a redox-active (NC)(2)C6H3-NO/(NC)(2)C6H3-NHOH (NOPH/NHOHPH) electrogenerated in situ from 4-nitrophthalonitrile (4-NPHN) is presented. The electrode modified with 4-NPHN showed an efficient electrocatalytic activity towards the oxidation of NADH with activation overpotential of 0.12 V vs. Ag/AgCl. The formation of all intermediate charge transfer complex is proposed for the charge transfer reaction between NADH and the 4-NPHN-resulting system. The second-order rate constant for electrocatalytic oxidation of NADH, kappa(obs), and the apparent Michaelis-Menten constant K-M, at pH 7.0 were evaluated with rotating disk electrode (RDE) experiments, giving 1.0 x 10(4) mol(-1) Ls(-1) and 2.7 x 10(-5) mol L-1, respectively. Employing the Koutecky-Levich approach indicated that the NADH oxidation reaction involves two electrons. The sensor provided a linear response range for NADH from 0.8 up to 8.5 mu mol L-1 with sensitivity, detection, quantification limits and time response of 0.50 mu A L mu mol(-1), 0.25 mu mol L-1, 0.82 mu mol L-1 and 0.1 s. respectively. The repeatability of the measurements with the same sensor and different sensors. evaluated in terms of relative standard deviation, were 4.1 and 5.0%, respectively, for n = 10. (C) 2008 Elsevier B.V. All rights reserved.
dc.description24
dc.description3
dc.description448
dc.description454
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPEAL
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionPADCT
dc.descriptionBNB
dc.descriptionRENORBIO
dc.descriptionGerman Academic Exchange Service (DAAD)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Advanced Technology
dc.publisherOxford
dc.publisherInglaterra
dc.relationBiosensors & Bioelectronics
dc.relationBiosens. Bioelectron.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subject4-nitrophthalonitrile
dc.subjectNADH
dc.subjectelectrocatalytic
dc.subjectmechanism and kinetics
dc.subjectCarbon-paste Electrodes
dc.subjectNicotinamide Adenine-dinucleotide
dc.subjectChemically Modified Electrodes
dc.subjectElectrocatalytic Oxidation
dc.subjectNiobium Oxide
dc.subjectCoenzymes
dc.subjectFad
dc.subjectDerivatives
dc.subjectMediators
dc.subjectQuinones
dc.titleElectrochemical investigations of the reaction mechanism and kinetics between NADH and redox-active (NC)(2)C6H3-NHOH/(NC)(2)C6H3-NO from 4-nitrophthalonitrile-(NC)(2)C6H3-NO2-modified electrode
dc.typeArtículos de revistas


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