An immobilized acetylcholinesterase flow-injection conductimetric system for the determination of paraoxon

dc.creatorRodrigues, TC
dc.creatorTubino, M
dc.creatorGodinho, OES
dc.creatorNeto, GD
dc.date1997
dc.dateJUN
dc.date2014-12-16T11:31:53Z
dc.date2015-11-26T16:54:27Z
dc.date2014-12-16T11:31:53Z
dc.date2015-11-26T16:54:27Z
dc.date.accessioned2018-03-28T23:41:44Z
dc.date.available2018-03-28T23:41:44Z
dc.identifierAnalytical Sciences. Japan Soc Analytical Chem, v. 13, n. 3, n. 423, n. 427, 1997.
dc.identifier0910-6340
dc.identifierWOS:A1997XE06800017
dc.identifier10.2116/analsci.13.423
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54414
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54414
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54414
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276860
dc.descriptionA flow-injection conductimetric system procedure is proposed for the determination of paraoxon. The method is based on the inhibition of immobilized acetylcholinesterase in controlled porous glass beads (CPG) with acetylcholine as substrate. The enzymatic reaction is inhibited by a diethyl-p-nitrophenylphosphate (paraoxon) solution. A 1,1'-trimethylene-bis(4-formylpyridinium bromide)dioxime solution was used to reactivate the enzyme. The correlation between the peak height, for a given acetylcholine concentration, is linear from 1.0x10(-7) to 5.0x10(-5) mol l(-1) of paraoxon; therefore, the quantitative limit of detection was about 1.0x10(-7) mol l(-1) of paraoxon. For semi-quantitative purposes, the limit can be considered to be about 1.0x10(-8) mol l(-1). The relative estimated standard deviation obtained using a 1.0x10(-3) mol l(-1) acetylcholine solution and a 5.0x10(-6) mol l(-1) paraoxon solution was 2.2% (n=8).
dc.description13
dc.description3
dc.description423
dc.description427
dc.languageen
dc.publisherJapan Soc Analytical Chem
dc.publisherTokyo
dc.publisherJapão
dc.relationAnalytical Sciences
dc.relationAnal. Sci.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectpesticide
dc.subjectacetylcholinesterase
dc.subjectacetylcholine
dc.subjectconductimetry
dc.subjectflow-injection
dc.subjectparaoxon
dc.subjectVolatile Acidity
dc.subjectGas-diffusion
dc.subjectPesticides
dc.subjectBiosensor
dc.titleAn immobilized acetylcholinesterase flow-injection conductimetric system for the determination of paraoxon
dc.typeArtículos de revistas


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