dc.creatordaSilva, LRD
dc.creatorGushikem, Y
dc.creatorKubota, LT
dc.date1996
dc.dateMAY 22
dc.date2014-12-16T11:31:43Z
dc.date2015-11-26T16:54:10Z
dc.date2014-12-16T11:31:43Z
dc.date2015-11-26T16:54:10Z
dc.date.accessioned2018-03-28T23:41:26Z
dc.date.available2018-03-28T23:41:26Z
dc.identifierColloids And Surfaces B-biointerfaces. Elsevier Science Bv, v. 6, n. 41763, n. 309, n. 315, 1996.
dc.identifier0927-7765
dc.identifierWOS:A1996UQ49000008
dc.identifier10.1016/0927-7765(95)01263-X
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69643
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69643
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69643
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276787
dc.descriptionHorseradish peroxidase (HRP) immobilized on TiO2 coated cellulose microfibers was used to study the catalytic oxidation of pyrogallol to purpurogallin in a flow injection (FI) system. The Michaelis-Menten constants for the enzymatic oxidation were determined for the immobilized enzyme, K-m=47 mM and V=22 mu mol min(-1), and compared with those obtained for free enzyme, K-m=11 mM and V=53 mu mol min(-1). The activity of the immobilized enzyme is reduced presumably due to its interaction with the oxide surface. However, the level of activity of the immobilized phase does not decrease with time under the conditions used in the FI system after various operation cycles.
dc.description6
dc.description41763
dc.description309
dc.description315
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationColloids And Surfaces B-biointerfaces
dc.relationColloid Surf. B-Biointerfaces
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectenzymatic activity
dc.subjectflow injection
dc.subjecthorseradish peroxidase
dc.subjecttitanium (iv) oxide coated cellulose
dc.subjectDecolorization
dc.subjectReactors
dc.subjectGlucose
dc.titleHorseradish peroxidase enzyme immobilized on titanium(iv) oxide coated cellulose microfibers: Study of the enzymatic activity by flow injection system
dc.typeArtículos de revistas


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