Exogenous SOD Mimetic Tempol Ameliorates the Early Retinal Changes Reestablishing the Redox Status in Diabetic Hypertensive Rats

dc.creatorRosales, MAB
dc.creatorSilva, KC
dc.creatorde Faria, JBL
dc.creatorde Faria, JML
dc.date2010
dc.dateAUG
dc.date2014-11-18T10:44:04Z
dc.date2015-11-26T16:54:01Z
dc.date2014-11-18T10:44:04Z
dc.date2015-11-26T16:54:01Z
dc.date.accessioned2018-03-28T23:41:16Z
dc.date.available2018-03-28T23:41:16Z
dc.identifierInvestigative Ophthalmology & Visual Science. Assoc Research Vision Ophthalmology Inc, v. 51, n. 8, n. 4327, n. 4336, 2010.
dc.identifier0146-0404
dc.identifierWOS:000280194100067
dc.identifier10.1167/iovs.09-4690
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66311
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66311
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66311
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276744
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionPURPOSE. The purpose of this study was to investigate the efficacy of tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), a superoxide dismutase mimetic, in preventing early retinal molecular changes in a model that combines hypertension and diabetes. METHODS. Four-week-old spontaneously hypertensive rats (SHR) were rendered diabetic by streptozotocin. Diabetic SHR rats (DM-SHR) were randomized to receive or not receive tempol treatment. After 20 days of induction of diabetes, the rats were euthanatized, and their retinas were collected. RESULTS. The early molecular markers of diabetic retinopathy (DR), glial fibrillary acidic protein, and fibronectin were evaluated by Western blot assays and showed an increase in DM-SHR compared with the SHR group. The oxidative balance, evaluated by superoxide production and nitric oxide end product levels estimated by a nitric oxide analyzer, and the counterpart antioxidative defense revealed an accentuated imbalance in DM-SHR compared with the SHR group. As a result, the product peroxynitrite, which was detected by immunohistochemistry for nitrotyrosine, was higher in the DM-SHR group. The retinal poly-ADP-ribose (PAR)-modified proteins, which reflect the activation of PAR polymerase (PARP), and the inducible nitric oxide synthase (iNOS) expressions were found to have increased in this group. Treatment with tempol reestablished the oxidative parameters and decreased the PAR-modified proteins, thus preventing extracellular matrix accumulation and glial reaction. CONCLUSIONS. The administration of tempol prevented oxidative damage, decreased iNOS levels, and ameliorated the activation of PARP in the retinas of diabetic hypertensive rats. Consequently, the early molecular markers of DR, such as glial reaction (glial fibrillary acidic protein [GFAP]) and extracellular matrix accumulation (fibronectin), were prevented in tempol-treated rats. (Invest Ophthalmol Vis Sci. 2010; 51:4327-4336) DOI:10.1167/iovs.09-4690
dc.descriptionO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.
dc.description51
dc.description8
dc.description4327
dc.description4336
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordination for the Improvement of Higher Education Personnel
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [05/58189-5, 08/54068-7]
dc.languageen
dc.publisherAssoc Research Vision Ophthalmology Inc
dc.publisherRockville
dc.publisherEUA
dc.relationInvestigative Ophthalmology & Visual Science
dc.relationInvest. Ophthalmol. Vis. Sci.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectEndothelial Growth-factor
dc.subjectFibrillary Acidic Protein
dc.subjectGalactose-fed Rats
dc.subjectNitric-oxide
dc.subjectOxidative Stress
dc.subjectPoly(adp-ribose) Polymerase
dc.subjectSuperoxide-dismutase
dc.subjectKappa-b
dc.subjectAngiotensin-ii
dc.subjectVitreous Fluid
dc.titleExogenous SOD Mimetic Tempol Ameliorates the Early Retinal Changes Reestablishing the Redox Status in Diabetic Hypertensive Rats
dc.typeArtículos de revistas


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