dc.creatorContesini, FJ
dc.creatorIbarguren, C
dc.creatorGrosso, CRF
dc.creatorCarvalho, PD
dc.creatorSato, HH
dc.date2012
dc.dateAPR 15
dc.date2014-07-30T18:05:50Z
dc.date2015-11-26T16:53:28Z
dc.date2014-07-30T18:05:50Z
dc.date2015-11-26T16:53:28Z
dc.date.accessioned2018-03-28T23:40:35Z
dc.date.available2018-03-28T23:40:35Z
dc.identifierJournal Of Biotechnology. Elsevier Science Bv, v. 158, n. 3, n. 137, n. 143, 2012.
dc.identifier0168-1656
dc.identifierWOS:000301352700010
dc.identifier10.1016/j.jbiotec.2012.01.012
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69914
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69914
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276590
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionTwo different techniques of glucosyltransferase immobilization were studied for the conversion of sucrose into isomaltulose. The optimum conditions for immobilization of Erwinia sp. glucosyltransferase onto Celite 545, determined using response surface methodology, was pH 4.0 and 170 U of glucosyltransferase/g of Celite 545. Using this conditions more than 60% conversion of sucrose into isomaltulose can be obtained. The immobilization of glucosyltransferase was also studied by its entrapment in microcapsules of low-methoxyl pectin and fat (butter and oleic acid). The non-lyophilized microcapsules of pectin, containing the enzyme and fat, showed higher glucosyltransferase activity, compared with lyophilized microcapsules containing enzyme plus fat, and also lyophilized microcapsules containing enzyme without fat addition. The non-lyophilized microcapsules of pectin containing the glucosyltransferase and fat, converted 30% of sucrose into isomaltulose in the first batch. However the conversion decreased to 5% at the 10th batch, indicating inactivation of the enzyme. (C) 2012 Elsevier B.V. All rights reserved.
dc.description158
dc.description3
dc.description137
dc.description143
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Biotechnology
dc.relationJ. Biotechnol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectGlucosyltransferase
dc.subjectIsomaltulose
dc.subjectTrehalulose
dc.subjectErwinia sp.
dc.subjectImmobilization
dc.subjectIsomaltulose Production
dc.subjectCells
dc.subjectPalatinose
dc.subjectSucrose
dc.subjectGlutaraldehyde
dc.subjectConversion
dc.subjectOptimization
dc.subjectAlginate
dc.subjectLipase
dc.titleImmobilization of glucosyltransferase from Erwinia sp using two different techniques
dc.typeArtículos de revistas


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