RNA relocation and persistence of nucleolus-like bodies at mitosis in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17

dc.creatorMello, MLS
dc.creatorLareef, MH
dc.creatorVidal, BC
dc.creatorRusso, J
dc.date2001
dc.date2014-07-30T18:28:06Z
dc.date2015-11-26T16:53:00Z
dc.date2014-07-30T18:28:06Z
dc.date2015-11-26T16:53:00Z
dc.date.accessioned2018-03-28T23:40:01Z
dc.date.available2018-03-28T23:40:01Z
dc.identifierAnalytical Cellular Pathology. Ios Press, v. 23, n. 41732, n. 137, n. 141, 2001.
dc.identifier0921-8912
dc.identifierWOS:000175765800004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71151
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71151
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276445
dc.descriptionRNA relocation and the incidence of nucleolus-like bodies accumulated during mitosis were studied cytochemically in benzo[a]pyrene (BP)-transformed human breast epithelial MCF-10F cells after microcell-mediated transfer of normal chromosomes 11 and 17. The changes resulting from the transfer of these two chromosomes in tumorigenic MCF-10F cells (BP1-E cell line) were examined, since alterations in these chromosomes are involved in the expression of the transformed and tumorigenic phenotypes in the MCF-10F cell series. In addition, the frequency of nucleolus-like bodies decreases drastically with transformation and tumorigenicity in MCF-10F cells, thus being conceivable that it would be affected in presence of normal chromosomes 11 or 17. The pattern of RNA relocation associated with the mitotic spindle did not vary in the cell lines analyzed. The introduction of chromosome 17 in BP1-E cells either decreased or did not affect the frequency of persistent nucleolus-like bodies. In contrast, in cells which received a normal chromosome 11, the frequency of nucleolus-like bodies was closer to that of non-transformed MCF-10F cells. These results suggest that a normal chromosome 11 but not chromosome 17 contributes to the maintenance of an RNA surplus which accumulates in nucleolus-like bodies during cell division of the human breast epithelial cells, at least in vitro. Some loci which were retained in the BP1-E cells which received a normal chromosome 11 are probably involved with the control of RNA transcript production. Figure 1 on http://www.esacp.org/acp/2001/23-3,4/mello.htm
dc.description23
dc.description41732
dc.description137
dc.description141
dc.languageen
dc.publisherIos Press
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationAnalytical Cellular Pathology
dc.relationAnal. Cell. Pathol.
dc.rightsfechado
dc.rightshttp://www.iospress.nl/service/authors/author-copyright-agreement/
dc.sourceWeb of Science
dc.subjectRNA
dc.subjectnucleolus-like bodies
dc.subjecthuman breast epithelial cells
dc.subjectmicrocell-mediated chromosome transfer
dc.subjectchromosome 11
dc.subjectchromosome 17
dc.subjectMicrosatellite Instability
dc.subjectRepair Deficiency
dc.subjectIn-vitro
dc.subjectCancer
dc.subjectTransformation
dc.titleRNA relocation and persistence of nucleolus-like bodies at mitosis in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17
dc.typeArtículos de revistas


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