Global analysis of the sugarcane microtranscriptome reveals a unique composition of small RNAs associated with axillary bud outgrowth

dc.creatorOrtiz-Morea, FA
dc.creatorVicentini, R
dc.creatorSilva, GFF
dc.creatorSilva, EM
dc.creatorCarrer, H
dc.creatorRodrigues, AP
dc.creatorNogueira, FTS
dc.date2013
dc.dateMAY
dc.date2014-07-30T17:48:01Z
dc.date2015-11-26T16:52:53Z
dc.date2014-07-30T17:48:01Z
dc.date2015-11-26T16:52:53Z
dc.date.accessioned2018-03-28T23:39:54Z
dc.date.available2018-03-28T23:39:54Z
dc.identifierJournal Of Experimental Botany. Oxford Univ Press, v. 64, n. 8, n. 2307, n. 2320, 2013.
dc.identifier0022-0957
dc.identifierWOS:000319433200016
dc.identifier10.1093/jxb/ert089
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68076
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68076
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276414
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAxillary bud outgrowth determines shoot architecture and is under the control of endogenous hormones and a fine-tuned gene-expression network, which probably includes small RNAs (sRNAs). Although it is well known that sRNAs act broadly in plant development, our understanding about their roles in vegetative bud outgrowth remains limited. Moreover, the expression profiles of microRNAs (miRNAs) and their targets within axillary buds are largely unknown. Here, we employed sRNA next-generation sequencing as well as computational and gene-expression analysis to identify and quantify sRNAs and their targets in vegetative axillary buds of the biofuel crop sugarcane (Saccharum spp.). Computational analysis allowed the identification of 26 conserved miRNA families and two putative novel miRNAs, as well as a number of trans-acting small interfering RNAs. sRNAs associated with transposable elements and protein-encoding genes were similarly represented in both inactive and developing bud libraries. Conversely, sequencing and quantitative reverse transcription-PCR results revealed that specific miRNAs were differentially expressed in developing buds, and some correlated negatively with the expression of their targets at specific stages of axillary bud development. For instance, the expression patterns of miR159 and its target GAMYB suggested that they may play roles in regulating abscisic acid-signalling pathways during sugarcane bud outgrowth. Our work reveals, for the first time, differences in the composition and expression profiles of diverse sRNAs and targets between inactive and developing vegetative buds that, together with the endogenous balance of specific hormones, may be important in regulating axillary bud outgrowth.
dc.description64
dc.description8
dc.description2307
dc.description2320
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [07/58289-5]
dc.descriptionCNPq [474635/2008-2]
dc.languageen
dc.publisherOxford Univ Press
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Experimental Botany
dc.relationJ. Exp. Bot.
dc.rightsfechado
dc.rightshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.sourceWeb of Science
dc.subjectmicroRNAs
dc.subjectphytormones
dc.subjectsmall RNAs
dc.subjectsugarcane
dc.subjectvegetative axillary buds
dc.subjectGene-expression
dc.subjectTransposable Elements
dc.subjectArabidopsis Micrornas
dc.subjectPlant Architecture
dc.subjectShoot
dc.subjectIdentification
dc.subjectBiogenesis
dc.subjectDormancy
dc.subjectSirnas
dc.subjectMaize
dc.titleGlobal analysis of the sugarcane microtranscriptome reveals a unique composition of small RNAs associated with axillary bud outgrowth
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución