Histological and functional renal alterations caused by Bothrops alternatus snake venom: Expression and activity of Na+/K+-ATPase

dc.creatorLinardi, A
dc.creatorSilva, TAARE
dc.creatorMiyabara, EH
dc.creatorFranco-Penteado, CF
dc.creatorCardoso, KC
dc.creatorBoer, PA
dc.creatorMoriscot, AS
dc.creatorGontijo, JAR
dc.creatorJoazeiro, PP
dc.creatorCollares-Buzato, CB
dc.creatorHyslop, S
dc.date2011
dc.dateSEP
dc.date2014-07-30T18:04:34Z
dc.date2015-11-26T16:52:44Z
dc.date2014-07-30T18:04:34Z
dc.date2015-11-26T16:52:44Z
dc.date.accessioned2018-03-28T23:39:43Z
dc.date.available2018-03-28T23:39:43Z
dc.identifierBiochimica Et Biophysica Acta-general Subjects. Elsevier Science Bv, v. 1810, n. 9, n. 895, n. 906, 2011.
dc.identifier0304-4165
dc.identifierWOS:000293932400012
dc.identifier10.1016/j.bbagen.2011.06.006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69860
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69860
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276366
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBackground: Acute renal failure is a serious complication of human envenoming by Bothrops snakes. The ion pump Na+/K+-ATPase has an important role in renal tubule function, where it modulates sodium reabsorption and homeostasis of the extracellular compartment. Here, we investigated the morphological and functional renal alterations and changes in Na+/K+-ATPase expression and activity in rats injected with Bothrops alternatus snake venom. Methods: Male Wistar rats were injected with venom (0.8 mg/kg, iv.) and renal function was assessed 6.24, 48 and 72 h and 7 days post-venom. The rats were then killed and renal Na+/K+-ATPase activity was assayed based on phosphate release from ATP; gene and protein expressions were assessed by real time PCR and immunofluorescence microscopy, respectively. Results: Venom caused lobulation of the capillary tufts, dilation of Bowman's capsular space. F-actin disruption in Bowman's capsule and renal tubule brush border, and deposition of collagen around glomeruli and proximal tubules that persisted seven days after envenoming. Enhanced sodium and potassium excretion, reduced proximal sodium reabsorption, and proteinuria were observed 6 h post-venom, followed by a transient decrease in the glomerular filtration rate. Gene and protein expressions of the Na+/K+-ATPase alpha(1) subunit were increased 6 h post-venom, whereas Na+/K+-ATPase activity increased 6 h and 24 h post-venom. Conclusions: Bothrops alternatus venom caused marked morphological and functional renal alterations with enhanced Na+/K+-ATPase expression and activity in the early phase of renal damage. General significance: Enhanced Na+/K+-ATPase activity in the early hours after envenoming may attenuate the renal dysfunction associated with venom-induced damage. (C) 2011 Elsevier B.V. All rights reserved.
dc.description1810
dc.description9
dc.description895
dc.description906
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationBiochimica Et Biophysica Acta-general Subjects
dc.relationBiochim. Biophys. Acta-Gen. Subj.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAcute renal failure
dc.subjectBothrops alternatus
dc.subjectCollagen deposition
dc.subjectF-actin
dc.subjectNa+/K+-ATPase
dc.subjectRenal function
dc.subjectExtensor Digitorum Longus
dc.subjectIschemia-induced Loss
dc.subjectLocal Tissue-damage
dc.subjectEpithelial Polarity
dc.subjectJararacussu Venom
dc.subjectAsper
dc.subjectKidney
dc.subjectRats
dc.subjectMetalloproteinases
dc.subjectMechanisms
dc.titleHistological and functional renal alterations caused by Bothrops alternatus snake venom: Expression and activity of Na+/K+-ATPase
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución