Effects of different sterilization methods on the morphology, mechanical properties, and cytotoxicity of chitosan membranes used as wound dressings

dc.creatorMarreco, PR
dc.creatorda Luz Moreira, P
dc.creatorGenari, SC
dc.creatorMoraes, AM
dc.date2004
dc.date42309
dc.date2014-11-18T08:01:02Z
dc.date2015-11-26T16:52:42Z
dc.date2014-11-18T08:01:02Z
dc.date2015-11-26T16:52:42Z
dc.date.accessioned2018-03-28T23:39:40Z
dc.date.available2018-03-28T23:39:40Z
dc.identifierJournal Of Biomedical Materials Research Part B-applied Biomaterials. Wiley-blackwell, v. 71B, n. 2, n. 268, n. 277, 2004.
dc.identifier1552-4973
dc.identifierWOS:000224846700007
dc.identifier10.1002/jbm.b.30081
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64139
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64139
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64139
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1276355
dc.descriptionThe aim of this work was to compare the effects induced by two different sterilization methods (exposure to gamma radiation or ethylene oxide) and an antiseptic technique (immersion in 70% ethanol aqueous solution) on the morphology, tensile strength, percentage of strain at break, and in vitro cytotoxicity to Vero cells on chitosan membranes designed for wound healing. Four distinct membrane compositions were evaluated, with chitosan, glycerol, and chitin used as components. Gamma radiation, in spite of being one of the most commonly employed sterilizing agents, negatively affected the morphology of membranes composed solely by chitosan as well as the percentage of strain at break of the chitosan-membranes containing glycerol on their composition. Moreover, its use affected the color of the chitosan membranes. The use of 70% ethanol aqueous solution did not change the chitosan-membrane characteristics significantly, but its use has limitations concerning the process scale up. With ethylene oxide (EtO), chitosan-membrane morphology, percentage of strain at break, and in vitro cytotoxicity to Vero cells were not significantly affected. The tensile strength of the membranes containing chitin were reduced after the treatment with ethylene oxide; however, the obtained values were comprised in the range verified for normal human skin. Therefore, because the final characteristics of the membranes treated with ethylene oxide are appropriate when considering their use as wound healing devices, and because this sterilization process is easily adjusted to use on an industrial scale, EtO can be considered the most adequate sterilizing agent for chitosan membranes. However, it should be considered that this chemical is associated with toxicity, flammability, and environmental risks, as well as with possible material contamination with ethylene oxide residues. (C) 2004 Wiley Periodicals, Inc.
dc.description71B
dc.description2
dc.description268
dc.description277
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationJournal Of Biomedical Materials Research Part B-applied Biomaterials
dc.relationJ. Biomed. Mater. Res. Part B
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectchitin
dc.subjectchitosan
dc.subjectwound dressing
dc.subjectbiomaterial
dc.subjectsterilization
dc.subjectcharacterization
dc.subjectIn-vitro
dc.subjectPhysical-properties
dc.subjectFilms
dc.subjectGrowth
dc.subjectChitin
dc.subjectFabrication
dc.subjectFibroblasts
dc.subjectManagement
dc.subjectResponses
dc.subjectPolymers
dc.titleEffects of different sterilization methods on the morphology, mechanical properties, and cytotoxicity of chitosan membranes used as wound dressings
dc.typeArtículos de revistas


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