IgG avidity in differential serodiagnosis of human strongyloidiasis active infection

dc.creatorGonzaga, HT
dc.creatorRibeiro, VS
dc.creatorFeliciano, ND
dc.creatorManhani, MN
dc.creatorSilva, DAO
dc.creatorUeta, MT
dc.creatorCosta-Cruz, JM
dc.date2011
dc.dateSEP 30
dc.date2014-07-30T18:00:25Z
dc.date2015-11-26T16:51:19Z
dc.date2014-07-30T18:00:25Z
dc.date2015-11-26T16:51:19Z
dc.date.accessioned2018-03-28T23:38:08Z
dc.date.available2018-03-28T23:38:08Z
dc.identifierImmunology Letters. Elsevier Science Bv, v. 139, n. 41671, n. 87, n. 92, 2011.
dc.identifier0165-2478
dc.identifierWOS:000293868700012
dc.identifier10.1016/j.imlet.2011.05.006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69208
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69208
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275960
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionIgG avidity assays have been developed for several parasitic diseases although there are no researches focused in strongyloidiasis diagnosis. Definitive diagnosis of strongyloidiasis is based on the presence of Strongyloides larvae in stool, but majority of cases involve low and irregular larval output. While limitations of serological assays for strongyloidiasis are well known, characteristics of persons who are misdiagnosed based on negative coproparasitological tests have been little explored. The aim of the present study was to evaluate the use of IgG avidity to detect patients with active strongyloidiasis and to characterize sources of disagreement between serology and coproparasitology. A total of 80 serum samples was analyzed, 40 from patients with Strongyloides larvae in stool (G1) and 40 from individuals with negative coproparasitology, but positive serology (G2). Serum samples were analyzed in an indirect IgG avidity ELISA using urea 6M in serial double dilutions from 1:80 to 1:2560. Avidity index (AI) was calculated to each serum dilution and analyzed as screening AI (serum dilution of 1:160) or mean AI of different serum dilutions that had a positive result. Statistical analyzes were performed by Mann-Whitney's (U) and Fisher's exact tests. At screening dilution, median of AI was 68% in G1 and 88% in G2 (P < 0.0001), whereas median of mean AI in G1 was 72% and in G2 94% (P < 0.0001), but there was no significant differences between both AI in each patient group. A cut off value established at AI of 75% demonstrated a significant difference between groups, with Cl sera showing AI <75% and G2 sera with AI > 75% (P < 0.0001). In conclusion, IgG avidity assays may distinguish active infection with Strongyloides stercoralis from suspect or serologically false positive cases. (C) 2011 Elsevier B.V. All rights reserved.
dc.description139
dc.description41671
dc.description87
dc.description92
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.descriptionUniversidade Federal de Uberlandia (UFU), Brazil
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationImmunology Letters
dc.relationImmunol. Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectIgG avidity ELISA
dc.subjectStrongyloidiasis
dc.subjectSerodiagnosis
dc.subjectStrongyloides stercoralis
dc.subjectStrongyloides venezuelensis
dc.subjectLinked-immunosorbent-assay
dc.subjectImmunoglobulin-g Avidity
dc.subjectStercoralis Infection
dc.subjectAcute Toxoplasmosis
dc.subjectDiagnosis
dc.subjectAntibodies
dc.subjectProteins
dc.subjectFraction
dc.subjectAntigen
dc.subjectCattle
dc.titleIgG avidity in differential serodiagnosis of human strongyloidiasis active infection
dc.typeArtículos de revistas


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