Entropy of Feulgen-stained 17-beta-estradiol-transformed human breast epithelial cells as assessed by restriction enzymes and image analysis

dc.creatorMello, MLS
dc.creatorRusso, P
dc.creatorRusso, J
dc.creatorVidal, BC
dc.date2009
dc.dateJUN
dc.date2014-11-18T04:16:11Z
dc.date2015-11-26T16:51:10Z
dc.date2014-11-18T04:16:11Z
dc.date2015-11-26T16:51:10Z
dc.date.accessioned2018-03-28T23:37:56Z
dc.date.available2018-03-28T23:37:56Z
dc.identifierOncology Reports. Spandidos Publ Ltd, v. 21, n. 6, n. 1483, n. 1487, 2009.
dc.identifier1021-335X
dc.identifierWOS:000266185900018
dc.identifier10.3892/or_00000378
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64907
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64907
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64907
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275914
dc.descriptionMCF-10F human breast epithelial cells when transformed with 17-beta-estradiol (E2) give rise to highly invasive C5 cells that generate adenocarcinomas in SCID mice. From these tumor, cell lines Such as C5-A6-T6 and C5-A8-T8 have been derived. Variable patterns of chromatin supraorganization have been demonstrated for these cells during the transformation/tumorigenesis progress, when assessing chromatin entropy by image analysis in Feulgenstained preparations. Since epigenetic dysregulation might contribute to the chromatin textural repatterning in transformed MCF-10F cells, the association of the variable chromatin packing states with global DNA methylation was investigated in these cells after their treatment with restriction enzymes followed by Feulgen staining and chromatin entropy evaluation by image analysis. The results indicate that although -C(m)CGG- sequences may affect chromatin supraorganization in some of the analyzed cell types (perhaps due to localized hypermethylation), not all the chromatin condensation patterns in these cells with transformation and/or tumorigenesis are associated with DNA methylation (e.g. E2 cells). Chromatin supraorganization remodeling in C5-A6-T6 and C5-A8-T8 cells may be attained by different mechanisms, with C5-A6-T6 chromatin packing states perhaps being associated with local DNA hypermethylation or other epigenetic factors, and C5-A8-T8 likely being associated with global DNA hypomethylation, as reported in the literature for other cell types. Thus, we assume that a variable epigenetic modulation affecting the higher-order packing states of chromatin in the estrogen-transformed MCF-10F cell model could be evident with the chromatin entropy study by image analysis.
dc.descriptionO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.
dc.description21
dc.description6
dc.description1483
dc.description1487
dc.languageen
dc.publisherSpandidos Publ Ltd
dc.publisherAthens
dc.publisherGrécia
dc.relationOncology Reports
dc.relationOncol. Rep.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjecthuman breast epithelial cells
dc.subject17-beta-estradiol
dc.subjecttumor-derived cells
dc.subjectDNA methylation
dc.subjectchromatin packing states
dc.subjectentropy
dc.subjectimage analysis
dc.subjectDna Methylation
dc.subjectMouse Hepatocytes
dc.subjectChromatin
dc.subjectTransformation
dc.subjectHeterochromatin
dc.subjectTumorigenesis
dc.subjectEndonucleases
dc.subjectChromosomes
dc.subjectDigestion
dc.subjectBinding
dc.titleEntropy of Feulgen-stained 17-beta-estradiol-transformed human breast epithelial cells as assessed by restriction enzymes and image analysis
dc.typeArtículos de revistas


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