Leishmanicidal activity of primary S-nitrosothiols against Leishmania major and Leishmania amazonensis: Implications for the treatment of cutaneous leishmaniasis

dc.creatorde Souza, GFP
dc.creatorYokoyama-Yasunaka, JKU
dc.creatorSeabra, AB
dc.creatorMiguel, DC
dc.creatorde Oliveira, MG
dc.creatorUliana, SRB
dc.date2006
dc.dateNOV
dc.date2014-11-18T04:09:07Z
dc.date2015-11-26T16:51:08Z
dc.date2014-11-18T04:09:07Z
dc.date2015-11-26T16:51:08Z
dc.date.accessioned2018-03-28T23:37:55Z
dc.date.available2018-03-28T23:37:55Z
dc.identifierNitric Oxide-biology And Chemistry. Academic Press Inc Elsevier Science, v. 15, n. 3, n. 209, n. 216, 2006.
dc.identifier1089-8603
dc.identifierWOS:000241419400005
dc.identifier10.1016/j.niox.2006.01.011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80846
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80846
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80846
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275906
dc.descriptionNitric oxide (NO) is considered a key molecule in the defense against intracellular pathogens, particularly Leishmania. The expression of inducible nitric oxide synthase and consequent production of NO by infected macrophages has been shown to correlate with leishmaniasis resistance in the murine model as well as in human patients. Nitric oxide donors have been used successfully in the treatment of cutaneous leishmaniasis in humans, although their mechanisms of action are not fully understood. In the present work, the dose-dependent cytotoxic effects of the NO-donors S-nitroso-N-acetyl-L-Cysteine (SNAC) and S-nitrosoglutathione (GSNO) against Leishmania were evaluated. GSNO inhibited the growth of Leishmania major and Leishmania amazonensis with in vitro 50% inhibitory concentrations (IC50) of 68.8 +/- 22.86 and 68.9 +/- 7.9 mu mol L-1, respectively. The IC50 for SNAC against L. major and L. amazonensis were, respectively, 54.6 +/- 8.3 and 181.6 +/- 12.5 mu mol L-1. The leishmanicidal activity of GSNO, but not of SNAC, was reversed by ascorbic acid (AA) and dithiothreitol (DTT), suggesting that the mechanism of action of GSNO is related to the transnitrosation of parasite proteins. These results demonstrate that SNAC and GSNO have leishmanicidal activity, and are thus potential therapeutic agents against cutaneous leishmaniasis. (c) 2006 Elsevier Inc. All rights reserved.
dc.description15
dc.description3
dc.description209
dc.description216
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationNitric Oxide-biology And Chemistry
dc.relationNitric Oxide-Biol. Chem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectnitric oxide
dc.subjectS-nitrosoglutathione
dc.subjectS-nitroso-N-acetyl-L-cysteine
dc.subjectcutaneous leishmaniasis
dc.subjectS-nitrosation
dc.subjectNitric-oxide Release
dc.subjectNo-donors
dc.subjectCysteine
dc.subjectNitrosylation
dc.subjectMacrophages
dc.subjectInhibition
dc.subjectMechanisms
dc.subjectNitrosoglutathione
dc.subjectInactivation
dc.subjectNitrosation
dc.titleLeishmanicidal activity of primary S-nitrosothiols against Leishmania major and Leishmania amazonensis: Implications for the treatment of cutaneous leishmaniasis
dc.typeArtículos de revistas


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