In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus

dc.creatorAnhe, GF
dc.creatorCaperuto, LC
dc.creatorPereira-Da-Silva, M
dc.creatorSouza, LC
dc.creatorHirata, AE
dc.creatorVelloso, LA
dc.creatorCipolla-Neto, J
dc.creatorCarvalho, CRO
dc.date2004
dc.dateAUG
dc.date2014-11-18T03:27:33Z
dc.date2015-11-26T16:50:18Z
dc.date2014-11-18T03:27:33Z
dc.date2015-11-26T16:50:18Z
dc.date.accessioned2018-03-28T23:37:03Z
dc.date.available2018-03-28T23:37:03Z
dc.identifierJournal Of Neurochemistry. Blackwell Publishing Ltd, v. 90, n. 3, n. 559, n. 566, 2004.
dc.identifier0022-3042
dc.identifierWOS:000222720500005
dc.identifier10.1111/j.1471-4159.2004.02514.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69424
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69424
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69424
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275686
dc.descriptionMelatonin is the pineal hormone that acts via a pertussis toxin-sensitive G-protein to inhibit adenylate cyclase. However, the intracellular signalling effects of melatonin are not completely understood. Melatonin receptors are mainly present in the suprachiasmatic nucleus (SCN) and pars tuberalis of both humans and rats. The SCN directly controls, amongst other mechanisms, the circadian rhythm of plasma glucose concentration. In this study, using immunoprecipitation and immunoblotting, we show that melatonin induces rapid tyrosine phosphorylation and activation of the insulin receptor beta-subunit tyrosine kinase (IR) in the rat hypothalamic suprachiasmatic region. Upon IR activation, tyrosine phosphorylation of IRS-1 was detected. In addition, melatonin induced IRS-1/PI(3)-kinase and IRS-1/SHP-2 associations and downstream AKT serine phosphorylation and MAPK (mitogen-activated protein kinase) phosphorylation, respectively. These results not only indicate a new signal transduction pathway for melatonin, but also a potential cross-talk between melatonin and insulin.
dc.description90
dc.description3
dc.description559
dc.description566
dc.languageen
dc.publisherBlackwell Publishing Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Neurochemistry
dc.relationJ. Neurochem.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectAKT
dc.subjectGPCR
dc.subjecthypothalamus
dc.subjectinsulin receptor
dc.subjectmelatonin
dc.subjecttyrosine phosphorylation
dc.subjectSuprachiasmatic Circadian Clock
dc.subjectProtein-kinase
dc.subjectSignal-transduction
dc.subjectBody-weight
dc.subjectPhosphatidylinositol 3-kinase
dc.subjectMolecular Pharmacology
dc.subjectTerminal Kinase
dc.subjectPhase Advances
dc.subjectGrowth-factor
dc.subjectDaily Rhythm
dc.titleIn vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus
dc.typeArtículos de revistas


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