Expression and purification of a small heat shock protein from the plant pathogen Xylella fastidiosa

dc.creatorAzzoni, AR
dc.creatorTada, SFS
dc.creatorRosselli, LK
dc.creatorPaula, DP
dc.creatorCatani, CF
dc.creatorSabino, AA
dc.creatorBarbosa, JARG
dc.creatorGuimaraes, BG
dc.creatorEberlin, MN
dc.creatorMedrano, FJ
dc.creatorSouza, AP
dc.date2004
dc.dateFEB
dc.date2014-11-18T03:09:50Z
dc.date2015-11-26T16:50:06Z
dc.date2014-11-18T03:09:50Z
dc.date2015-11-26T16:50:06Z
dc.date.accessioned2018-03-28T23:36:51Z
dc.date.available2018-03-28T23:36:51Z
dc.identifierProtein Expression And Purification. Academic Press Inc Elsevier Science, v. 33, n. 2, n. 297, n. 303, 2004.
dc.identifier1046-5928
dc.identifierWOS:000188414200017
dc.identifier10.1016/j.pep.2003.10.007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66455
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66455
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66455
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275637
dc.descriptionThe small heat shock proteins (smHSPs) belong to a family of proteins that function as molecular chaperones by preventing protein aggregation and are also known to contain a conserved region termed a-crystallin domain. Here, we report the expression, purification, and partial characterization of a novel smHSP (HSP17.9) from the phytopathogen Xylella fastidiosa, causal agent of the citrus variegated chlorosis (CVC). The gene was cloned into a pET32-Xa/LIC vector to over-express the protein coupled with fusion tags in Escherichia coli BL21(DE3). The expressed HSP17.9 was purified by immobilized metal affinity chromatography (IMAC) and had its identity determined by mass spectrometry (MALDI-TOF). The correct folding of the purified recombinant protein was verified by circular dichroism spectroscopy. Finally, the HSP17.9 protein also proved to efficiently prevent induced aggregation of insulin, strongly indicating a chaperone-like activity. (C) 2003 Elsevier Inc. All rights reserved.
dc.description33
dc.description2
dc.description297
dc.description303
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationProtein Expression And Purification
dc.relationProtein Expr. Purif.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectsmall heat shock protein
dc.subjectXylella fastidiosa smHSP
dc.subjectexpression and purification
dc.subjectAlpha-crystallin
dc.titleExpression and purification of a small heat shock protein from the plant pathogen Xylella fastidiosa
dc.typeArtículos de revistas


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