Operational intensification by direct product sequestration from cell disruptates - Application of a pellicular adsorbent in a mechanically integrated disruption-fluidised bed adsorption process

dc.creatorJahanshahi, M
dc.creatorSun, Y
dc.creatorSantos, E
dc.creatorPacek, A
dc.creatorFranco, TT
dc.creatorNienow, A
dc.creatorLyddiatt, A
dc.date2002
dc.dateOCT 20
dc.date2014-11-18T03:04:31Z
dc.date2015-11-26T16:50:05Z
dc.date2014-11-18T03:04:31Z
dc.date2015-11-26T16:50:05Z
dc.date.accessioned2018-03-28T23:36:50Z
dc.date.available2018-03-28T23:36:50Z
dc.identifierBiotechnology And Bioengineering. John Wiley & Sons Inc, v. 80, n. 2, n. 201, n. 212, 2002.
dc.identifier0006-3592
dc.identifierWOS:000178152700009
dc.identifier10.1002/bit.10360
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69487
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69487
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69487
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275629
dc.descriptionA novel prototype adsorbent, designed for intensified fluidised bed adsorption processes, was assembled by the emulsification coating of 4% (w/v) porous agarose upon a zirconia-silica solid core. The adsorbent, designated ZSA (particle density 1.75 g/ml, maximum pellicle depth 40 mum), was subjected to physical and biochemical comparison with the performance of two commercial adsorbents (Streamline and Macrosorb K4AX). Bed expansion qualities and hydrodynamic characteristics (N, D-axl, and B-o) of ZSA demonstrated a marked robustness in the face of elevated velocities (up to 550 cm/h) and biomass loading (up to 30% (ww/v)) disrupted yeast cells. Cibracron Blue derivatives of the pellicular prototype (ZSA-CB), evaluated in the batch and fluidised bed recovery of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from unclarified yeast disruptates, exhibited superior capacities and adsorption/desorption performance to the commercial derivatives. These advanced physical and biochemical properties facilitated a demonstration of the direct, mechanical coupling of bead-milling and fluidised bed adsorption in a fully integrated process for the accelerated recovery of G3PDH from yeast. The generic application of such pellicular adsorbents and integrated processes to the recovery of labile, intracellular products is discussed. (C) 2002 Wiley Periodicals, Inc.
dc.description80
dc.description2
dc.description201
dc.description212
dc.languageen
dc.publisherJohn Wiley & Sons Inc
dc.publisherHoboken
dc.publisherEUA
dc.relationBiotechnology And Bioengineering
dc.relationBiotechnol. Bioeng.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectpellicular adsorbents
dc.subjectfluidised bed adsorption
dc.subjectprocess intensification
dc.subjectprocess integration
dc.subjectglyceralclehyde 3-phosphate clehydrogenase
dc.subjectyeast bioprocessing
dc.subjectExpanded Beds
dc.subjectRecovery
dc.subjectPurification
dc.subjectProteins
dc.titleOperational intensification by direct product sequestration from cell disruptates - Application of a pellicular adsorbent in a mechanically integrated disruption-fluidised bed adsorption process
dc.typeArtículos de revistas


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