Effect of sodium diclofenac on serum and tissue concentration of amoxicillin and on staphylococcal infection

dc.creatorGroppo, FC
dc.creatorSimoes, RP
dc.creatorRamacciato, JC
dc.creatorRehder, V
dc.creatorde Andrade, ED
dc.creatorMattos, TR
dc.date2004
dc.dateJAN
dc.date2014-11-18T01:48:54Z
dc.date2015-11-26T16:49:33Z
dc.date2014-11-18T01:48:54Z
dc.date2015-11-26T16:49:33Z
dc.date.accessioned2018-03-28T23:36:18Z
dc.date.available2018-03-28T23:36:18Z
dc.identifierBiological & Pharmaceutical Bulletin. Pharmaceutical Soc Japan, v. 27, n. 1, n. 52, n. 55, 2004.
dc.identifier0918-6158
dc.identifierWOS:000187745600009
dc.identifier10.1248/bpb.27.52
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64281
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64281
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64281
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275491
dc.descriptionThe effect of sodium diclofenac on serum and tissue amoxicillin concentration as well as their effect against staphylococcal infection was observed. Four polyurethane sponges were placed in the back of thirty rats. After 14 d, two granulomatous tissues received 0.5 ml of 10(8) cfu/ml (Staphylococcus aureus). Two days later, the rats were divided into five groups: group I received amoxicillin 50 mg/kg/P.o., group 2 received amoxicillin 25 mg/kg/p. o., group 3 received sodium diclofenac 2.5 mg/kg/i.m. and amoxicillin 50 mg/kg/p. o., group 4 received sodium diclofenac 2.5 mg/kg/i.m., and group 5 (control group) received NaCl 1 ml/p.o. After six hours of drug administration, blood serum (10 mul) and noninfected granulomatous tissues were placed on Mueller-Hinton agar inoculated with 108 cfu/ml (S. aureus). Infected tissues were dispersed in a sonic system and were spread (10 mul) on salt mannitol agar. Microorganisms were counted and the inhibition zones were measured after 18 h of incubation at 37 degreesC. Amoxicillin tissue concentration was 6.27 mug/g for group 1, 2.18 mug/g for group 2, and 0.72 mug/g for group 3. The serum concentrations were 11.56 mug/ml for group 1, 5.36 mug/ml for group 2, and 1.34 mug/ml for group 3. No differences were observed among group 1, 2, and 3 regarding staphylococci counts (Kruskall-Wallis test p>0.05). Group 4 reduced (p<0.05) staphylococci counts comparing to group 5. It was concluded that sodium diclofenac reduced serum and tissue amoxicillin concentration and, even in large doses, amoxicillin was not effective in eradicating the staphylococcal infection after 6 h of administration.
dc.description27
dc.description1
dc.description52
dc.description55
dc.languageen
dc.publisherPharmaceutical Soc Japan
dc.publisherTokyo
dc.publisherJapão
dc.relationBiological & Pharmaceutical Bulletin
dc.relationBiol. Pharm. Bull.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectamoxicillin
dc.subjectsodium diclofenac
dc.subjectStaphylococcus aureus
dc.subjectstaphylococcal infection
dc.subjectPerformance Liquid-chromatography
dc.subjectPharmacokinetics
dc.subjectBioavailability
dc.subjectAbsorption
dc.subjectStability
dc.subjectPotassium
dc.subjectBioassay
dc.subjectPlasma
dc.subjectAcid
dc.titleEffect of sodium diclofenac on serum and tissue concentration of amoxicillin and on staphylococcal infection
dc.typeArtículos de revistas


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