Osteoblastic potency of bone marrow cells cultivated on functionalized biometals with cyclic RGD-peptide

dc.creatorJager, M
dc.creatorBoge, C
dc.creatorJanissen, R
dc.creatorRohrbeck, D
dc.creatorHulsen, T
dc.creatorLensing-Hohn, S
dc.creatorKrauspe, R
dc.creatorHerten, M
dc.date2013
dc.dateOCT
dc.date2014-07-30T18:03:48Z
dc.date2015-11-26T16:49:28Z
dc.date2014-07-30T18:03:48Z
dc.date2015-11-26T16:49:28Z
dc.date.accessioned2018-03-28T23:36:12Z
dc.date.available2018-03-28T23:36:12Z
dc.identifierJournal Of Biomedical Materials Research Part A. Wiley-blackwell, v. 101, n. 10, n. 2905, n. 2914, 2013.
dc.identifier1549-3296
dc.identifierWOS:000323648300016
dc.identifier10.1002/jbm.a.34590
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69802
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69802
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275467
dc.descriptionThe fixation of cementless endoprostheses requires excellent fixation at the bone implant interface. Although the surface structures of these implants are designed to promote osteoblastic differentiation, poor bone quality may prevent or delay osseointegration. There is evidence that RGD peptides known as recognition motifs for various integrins, promote cellular adhesion, influence cellular proliferation, and differentiation of local cells. In this study, five different metal surfaces were analyzed: Sandblasted (TiSa) and polished (TiPol) Ti6Al4V, porocoated (CCPor) and polished (CCPol) cobalt chrome and polished stainless steel (SS) were coated by ethanol amine and poly(ethylene glycol) to attach covalently RGD peptides. Human mesenchymal stromal cells of healthy donors were cultivated onto prior functionalized metal surfaces for 14 days without osteogenic stimulation. Cell proliferation and differentiation were quantitatively evaluated for native (I), NaOH pre-activated (II), NaOH pre-activated, and PEG-coated (III) as well as for RGD (IV) coated surfaces. The RGD immobilization efficiency was analyzed by epi-fluorescence spectroscopy, cell morphology was documented by light and scanning electron microscopy. The RGD-binding efficiency was TiSa > TiPol > SS > CCPor > CCPol. RGD coated surfaces showed the highest average cell proliferation on CCPol > SS > CCPor > TiSa TiPol, whereas cellular differentiation mostly correlated with the observed proliferation results, such as CCPol > TiSa > SS > CCPor > TiPol. Considering statistical analyses (significance level of = 0.05), the RGD-coating of all biometals in comparison and in respect of their particular controls showed no significant improvement in cellular proliferation and osteoblastic differentiation. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2905-2914, 2013.
dc.description101
dc.description10
dc.description2905
dc.description2914
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationJournal Of Biomedical Materials Research Part A
dc.relationJ. Biomed. Mater. Res. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectRGD peptide
dc.subjectbone marrow
dc.subjectmesenchymal stem cells
dc.subjectosteoblast
dc.subjectendoprosthesis
dc.subjectCoated Titanium Implants
dc.subjectFormation In-vivo
dc.subjectCartilage Regeneration
dc.subjectHighly Potent
dc.subjectAdhesion
dc.subjectDifferentiation
dc.subjectSurfaces
dc.subjectFibronectin
dc.subjectImmobilization
dc.subjectCollagen
dc.titleOsteoblastic potency of bone marrow cells cultivated on functionalized biometals with cyclic RGD-peptide
dc.typeArtículos de revistas


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