In Vitro Photodynamic Antimicrobial Chemotherapy in Dentine Contaminated by Cariogenic Bacteria

dc.creatorMelo, MAS
dc.creatorde-Paula, DM
dc.creatorLima, JPM
dc.creatorBorges, FMC
dc.creatorSteiner-Oliveira, C
dc.creatorNobre-dos-Santos, M
dc.creatorZanin, ICJ
dc.creatorBarros, EB
dc.creatorRodrigues, LKA
dc.date2010
dc.dateJUN
dc.date2014-11-18T00:28:34Z
dc.date2015-11-26T16:48:13Z
dc.date2014-11-18T00:28:34Z
dc.date2015-11-26T16:48:13Z
dc.date.accessioned2018-03-28T23:34:36Z
dc.date.available2018-03-28T23:34:36Z
dc.identifierLaser Physics. Maik Nauka/interperiodica/springer, v. 20, n. 6, n. 1504, n. 1513, 2010.
dc.identifier1054-660X
dc.identifierWOS:000280661400040
dc.identifier10.1134/S1054660X10110174
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69403
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69403
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69403
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275072
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe development of a method to ensure bacterial-free substrates without extensive cavity preparation would be highly useful to dentistry, since there is no currently available effective method for killing residual bacteria in dentinal tissue. This randomized in vitro study determined parameters for using toluidine blue O (TBO) with a light-emitting diode (LED) for dentine caries disinfection and monitored intrapulpal/periodontal temperatures during irradiation. Occlusal human dentine slabs were immersed in Streptococcus mutans culture for demineralization induction. Slabs were allocated to 10 groups (n - 15), which were treated with 0.1 mg ml(-1) TBO with 5 min of incubation time or 0.9% NaCl solution for 5, 10 or 15 min, and submitted or not to irradiation for 5, 10 or 15 min (47, 94, and 144 J/cm(2)). Before and after treatments, dentine samples were analyzed with regard to S. mutans counts. In whole teeth, temperature in pulp and periodontium was measured by thermocouples during irradiation. Kruskal-Wallis/Student-Newman-Keuls, and ANOVA/Tukey test were respectively utilized to compare log reductions and temperature rises between groups. Bacterial reduction was observed when dentine was exposed to both TBO and LED at all irradiation times, as well as to LED alone for 10 and 15 min. Temperature increases lower than 2 degrees C were observed for either pulp or periodontium. Concluding, LED combined with TBO is a safe and effective approach for dentine caries disinfection. Nevertheless, additional studies should be conducted to determine the influence of the irradiation in S. mutans viability in dentinal surface/tubules.
dc.description20
dc.description6
dc.description1504
dc.description1513
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [620160/2006-3, 564387/2008-8]
dc.languageen
dc.publisherMaik Nauka/interperiodica/springer
dc.publisherNew York
dc.publisherEUA
dc.relationLaser Physics
dc.relationLaser Phys.
dc.rightsaberto
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectToluidine-blue-o
dc.subjectStreptococcus-mutans
dc.subjectDiode-laser
dc.subjectOral Bacteria
dc.subjectTherapy
dc.subjectLight
dc.subjectBiofilms
dc.subjectPhotosensitization
dc.subjectViability
dc.subjectCollagen
dc.titleIn Vitro Photodynamic Antimicrobial Chemotherapy in Dentine Contaminated by Cariogenic Bacteria
dc.typeArtículos de revistas


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