Effects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions

dc.creatorFerreira, MC
dc.creatorBrito, VN
dc.creatorGameiro, J
dc.creatorCosta, MRSN
dc.creatorVasconcellos, ECC
dc.creatorCruz-Hofling, MA
dc.creatorVerinaud, L
dc.date2006
dc.dateAUG 1
dc.date2014-11-17T22:57:47Z
dc.date2015-11-26T16:47:24Z
dc.date2014-11-17T22:57:47Z
dc.date2015-11-26T16:47:24Z
dc.date.accessioned2018-03-28T23:33:33Z
dc.date.available2018-03-28T23:33:33Z
dc.identifierJournal Of Photochemistry And Photobiology B-biology. Elsevier Science Sa, v. 84, n. 2, n. 141, n. 149, 2006.
dc.identifier1011-1344
dc.identifierWOS:000239299800008
dc.identifier10.1016/j.jphotobiol.2006.02.011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64961
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64961
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64961
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274850
dc.descriptionParacoccidioidomycosis (PCM) is the most prevalent human mycosis in Latin America. The infection is thought to take place firstly in the lungs and then may disseminate to other organs and tissues. Treatment by currently available antifungals is lengthy, the drugs may have undesirable side effects, and some are costly. Occasional resistant strains of Paracoccidioides brasiliensis, the causative agent of PCM, have been reported. So, the search for more efficient treatments or adjuvant therapies has to be continued. In this work, we evaluated the effects of HeNe laser irradiation on cutaneous inflammatory lesions caused by the inoculation of 5 x 10(6)/0.1 ml yeasts cells into the back footpad of Balb/c mice. HeNe irradiation (lambda = 632.8 nm, 3 mW, incident energy of 3 J/cm(2)) was applied at days 7, 8 and 9 postinfection and histological and immunohistochemical analysis were done. Unirradiated animals were used as controls. The results showed that laser-treated mice presented reduction of footpad edema, faster cutaneous wound healing, confluent granuloma, diffuse- and more loosely distributed immunolabeling for TNF-alpha, enhanced labeling of IFN-gamma and any P. brasiliensis form detected, whereas multiple viable fungi were seen in diffuse widespread granulomas obtained from non-treated mice foot-pad. Fungi that were harvested from laser-treated animals presented no capability of growth in vitro as compared to those obtained from non-treated mice. We conclude that HeNe laser irradiation was able to inhibit the progress of inflammatory local reaction produced by P. brasiliensis infection and influence local cytokines production. We suggest that this treatment modality can be a useful coadjuvant tool to be combined with antifungal agents in the treatment of PCM ulcerations. The mechanisms involved in laser therapy of PCM lesions need further investigation. (c) 2006 Elsevier B.V. All rights reserved.
dc.description84
dc.description2
dc.description141
dc.description149
dc.languageen
dc.publisherElsevier Science Sa
dc.publisherLausanne
dc.publisherSuíça
dc.relationJournal Of Photochemistry And Photobiology B-biology
dc.relationJ. Photochem. Photobiol. B-Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectlow level laser therapy
dc.subjectHeNe laser
dc.subjectParacoccidioides brasiliensis
dc.subjectparacoccidioidomycosis
dc.subjectHelium-neon Laser
dc.subjectChemokine
dc.subjectBrasiliensis
dc.subjectInfection
dc.subjectCells
dc.subjectGamma
dc.subjectMice
dc.titleEffects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions
dc.typeArtículos de revistas


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