DNA and graphene as a new efficient platform for entrapment of methylene blue (MB): Studies of the electrocatalytic oxidation of beta-nicotinamide adenine dinucleotide

dc.creatorFerreira, GMM
dc.creatorde Oliveira, FM
dc.creatorLeite, FRF
dc.creatorMaroneze, CM
dc.creatorKubota, LT
dc.creatorDamos, FS
dc.creatorLuz, RDS
dc.date2013
dc.date11263
dc.date2014-07-30T14:49:19Z
dc.date2015-11-26T16:47:18Z
dc.date2014-07-30T14:49:19Z
dc.date2015-11-26T16:47:18Z
dc.date.accessioned2018-03-28T23:33:24Z
dc.date.available2018-03-28T23:33:24Z
dc.identifierElectrochimica Acta. Pergamon-elsevier Science Ltd, v. 111, n. 543, n. 551, 2013.
dc.identifier0013-4686
dc.identifier1873-3859
dc.identifierWOS:000329531100070
dc.identifier10.1016/j.electacta.2013.08.037
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62390
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62390
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274811
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe modification of glassy carbon (GC) electrode with deoxyribonucleic acid (DNA) and graphene is utilized as a new efficient platform for entrapment of methylene blue (MB). Electrochemical and electroanalytical properties of the modified electrode (DNA/graphene/MB) were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometry techniques. Cyclic voltammetric results indicated the excellent electrocatalytic activity of the resulting electrode toward oxidation of beta-nicotinamide adenine dinucleotide (NADH) at reduced overpotential (0.1 V vs. Ag/AgCl). It has been found that the DNA/graphene/MB modification has significantly enhanced the effective electrode response toward NADH oxidation. Cyclic voltammety and rotating disk electrode (RDE) experiments indicated that the NADH oxidation reaction involves two electrons and an electrocatalytic rate constant (k(obs)) of 1.75 x 10(6) mol(-1) L s(-1). The electrochemical sensor presented better performance in 0.1 mol L-1 phosphate buffer at pH 7.0. Other experimental parameters, such as the DNA, graphene, MB concentrations and the applied potential were optimized. Under optimized conditions, a linear response range from 10 mu mol L-1 to 1.50 mmol L-1 was obtained with a sensitivity of 12.75 mu A L mu mol(-1). The detection and quantification limits for NADH determination were 1.0 mu mol L-1 and 3.3 mu mol L-1, respectively. (C) 2013 Elsevier Ltd. All rights reserved.
dc.description111
dc.description543
dc.description551
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionRede Mineira de Quimica
dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.descriptionInstituto Nacional de Ciencia e Tecnologia em Bioanalitica
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationElectrochimica Acta
dc.relationElectrochim. Acta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectDNA
dc.subjectGraphene
dc.subjectMethylene blue
dc.subjectNADH
dc.subjectGlassy-carbon Electrode
dc.subjectLow-potential Detection
dc.subjectDirect Electrochemistry
dc.subjectAqueous Dispersions
dc.subjectGraphite-electrodes
dc.subjectNadh Oxidation
dc.subjectLabel-free
dc.subjectOxide
dc.subjectNanotubes
dc.subjectNanocomposite
dc.titleDNA and graphene as a new efficient platform for entrapment of methylene blue (MB): Studies of the electrocatalytic oxidation of beta-nicotinamide adenine dinucleotide
dc.typeArtículos de revistas


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